2yt4

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(New page: 200px<br /> <applet load="2yt4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2yt4, resolution 2.60&Aring;" /> '''Crystal structure o...)
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Revision as of 21:38, 12 November 2007

Image:2yt4.gif

2yt4, resolution 2.60Å

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Crystal structure of human DGCR8 core

Overview

A complex of Drosha with DGCR8 (or its homolog Pasha) cleaves primary, microRNA (pri-miRNA) substrates into precursor miRNA and initiates the, microRNA maturation process. Drosha provides the catalytic site for this, cleavage, whereas DGCR8 or Pasha provides a frame for anchoring substrate, pri-miRNAs. To clarify the molecular basis underlying recognition of, pri-miRNA by DGCR8 and Pasha, we determined the crystal structure of the, human DGCR8 core (DGCR8S, residues 493-720). In the structure, the two, double-stranded RNA-binding domains (dsRBDs) are arranged with pseudo, two-fold symmetry and are tightly packed against the C-terminal helix. The, H2 helix in each dsRBD is important for recognition of pri-miRNA, substrates. This structure, together with fluorescent resonance energy, transfer and mutational analyses, suggests that the DGCR8 core recognizes, pri-miRNA in two possible orientations. We propose a model for DGCR8's, recognition of pri-miRNA.

About this Structure

2YT4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human DGCR8 core., Sohn SY, Bae WJ, Kim JJ, Yeom KH, Kim VN, Cho Y, Nat Struct Mol Biol. 2007 Sep;14(9):847-53. Epub 2007 Aug 19. PMID:17704815

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