2j41

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[[Image:2j41.jpg|left|200px]]
[[Image:2j41.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2j41 |SIZE=350|CAPTION= <scene name='initialview01'>2j41</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_2j41", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+C+1198'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+B+1206'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+D+1207'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+A+1198'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+D+1208'>AC5</scene>, <scene name='pdbsite=AC6:K+Binding+Site+For+Residue+B+1207'>AC6</scene>, <scene name='pdbsite=AC7:K+Binding+Site+For+Residue+C+1199'>AC7</scene>, <scene name='pdbsite=AC8:K+Binding+Site+For+Residue+D+1209'>AC8</scene>, <scene name='pdbsite=AC9:5gp+Binding+Site+For+Residue+C+1200'>AC9</scene>, <scene name='pdbsite=BC1:5gp+Binding+Site+For+Residue+D+1210'>BC1</scene> and <scene name='pdbsite=BC2:5gp+Binding+Site+For+Residue+B+1208'>BC2</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=5GP:GUANOSINE-5&#39;-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Guanylate_kinase Guanylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.8 2.7.4.8] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2j41| PDB=2j41 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j41 OCA], [http://www.ebi.ac.uk/pdbsum/2j41 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j41 RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF STAPHYLOCOCCUS AUREUS GUANYLATE MONOPHOSPHATE KINASE'''
'''CRYSTAL STRUCTURE OF STAPHYLOCOCCUS AUREUS GUANYLATE MONOPHOSPHATE KINASE'''
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[[Category: Omari, K El.]]
[[Category: Omari, K El.]]
[[Category: Stammers, D K.]]
[[Category: Stammers, D K.]]
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[[Category: atp-binding]]
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[[Category: Atp-binding]]
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[[Category: gmk]]
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[[Category: Gmk]]
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[[Category: gmp]]
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[[Category: Gmp]]
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[[Category: kinase]]
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[[Category: Kinase]]
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[[Category: nucleotide-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: staphylococcus aureus]]
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[[Category: Staphylococcus aureus]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:18:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:52:33 2008''
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Revision as of 05:18, 4 May 2008

Image:2j41.jpg

Template:STRUCTURE 2j41

CRYSTAL STRUCTURE OF STAPHYLOCOCCUS AUREUS GUANYLATE MONOPHOSPHATE KINASE


Overview

Nucleotide monophosphate kinases (NMPKs) are potential antimicrobial drug targets owing to their role in supplying DNA and RNA precursors. The present work reports the crystal structure of Staphylococcus aureus guanylate monophosphate kinase (SaGMK) at 1.9 A resolution. The structure shows that unlike most GMKs SaGMK is dimeric, confirming the role of the extended C-terminus in dimer formation as first observed for Escherichia coli GMK (EcGMK). One of the two SaGMK dimers within the crystal asymmetric unit has two monomers in different conformations: an open form with a bound sulfate ion (mimicking the beta-phosphate of ATP) and a closed form with bound GMP and sulfate ion. GMP-induced domain movements in SaGMK can thus be defined by comparison of these conformational states. Like other GMKs, the binding of GMP firstly triggers a partial closure of the enzyme, diminishing the distance between the GMP-binding and ATP-binding sites. In addition, the closed structure shows the presence of a potassium ion in contact with the guanine ring of GMP. The potassium ion appears to form an integral part of the GMP-binding site, as the Tyr36 side chain has significantly moved to form a metal ion-ligand coordination involving the lone pair of the side-chain O atom. The potassium-binding site might also be exploited in the design of novel inhibitors.

About this Structure

2J41 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

Structure of Staphylococcus aureus guanylate monophosphate kinase., El Omari K, Dhaliwal B, Lockyer M, Charles I, Hawkins AR, Stammers DK, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt, 10):949-53. Epub 2006 Sep 19. PMID:17012781 Page seeded by OCA on Sun May 4 08:18:05 2008

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