2j7m
From Proteopedia
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[[Image:2j7m.jpg|left|200px]] | [[Image:2j7m.jpg|left|200px]] | ||
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'''CHARACTERIZATION OF A FAMILY 32 CBM''' | '''CHARACTERIZATION OF A FAMILY 32 CBM''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J7M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. This structure supersedes the now removed PDB entry | + | 2J7M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2j1f 2j1f]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7M OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Boraston, A B.]] | [[Category: Boraston, A B.]] | ||
[[Category: Ficko-Blean, E.]] | [[Category: Ficko-Blean, E.]] | ||
| - | [[Category: | + | [[Category: Carbohydrate binding]] |
| - | [[Category: | + | [[Category: Cbm]] |
| - | [[Category: | + | [[Category: Glycoside hydrolase]] |
| - | [[Category: | + | [[Category: H-trisaccharide]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
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Revision as of 05:27, 4 May 2008
CHARACTERIZATION OF A FAMILY 32 CBM
Overview
Clostridium perfringens is a notable colonizer of the human gastrointestinal tract. This bacterium is quite remarkable for a human pathogen by the number of glycoside hydrolases found in its genome. The modularity of these enzymes is striking as is the frequent occurrence of modules having amino acid sequence identity with family 32 carbohydrate-binding modules (CBMs), often referred to as F5/8 domains. Here we report the properties of family 32 CBMs from a C. perfringens N-acetyl-beta-hexosaminidase. Macroarray, UV difference, and isothermal titration calorimetry binding studies indicate a preference for the disaccharide LacNAc (beta-d-galactosyl-1,4-beta-d-N-acetylglucosamine). The molecular details of the interaction of this CBM with galactose, LacNAc, and the type II blood group H-trisaccharide are revealed by x-ray crystallographic studies at resolutions of 1.49, 2.4, and 2.3 A, respectively.
About this Structure
2J7M is a Single protein structure of sequence from Clostridium perfringens. This structure supersedes the now removed PDB entry 2j1f. Full crystallographic information is available from OCA.
Reference
The interaction of a carbohydrate-binding module from a Clostridium perfringens N-acetyl-beta-hexosaminidase with its carbohydrate receptor., Ficko-Blean E, Boraston AB, J Biol Chem. 2006 Dec 8;281(49):37748-57. Epub 2006 Sep 21. PMID:16990278 Page seeded by OCA on Sun May 4 08:27:54 2008
