2jb5

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[[Image:2jb5.jpg|left|200px]]
[[Image:2jb5.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2jb5 |SIZE=350|CAPTION= <scene name='initialview01'>2jb5</scene>, resolution 2.8&Aring;
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The line below this paragraph, containing "STRUCTURE_2jb5", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:T5c+Binding+Site+For+Chain+H'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=T5C:2-{(1E,3Z,5E,7E)-7-[3,3-DIMETHYL-5-SULFO-1-(2-SULFOETHYL)-1,3-DIHYDRO-2H-INDOL-2-YLIDENE]-4-METHYLHEPTA-1,3,5-TRIEN-1-YL}-3,3-DIMETHYL-5-SULFO-1-(2-SULFOETHYL)-3H-INDOLIUM'>T5C</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2jb5| PDB=2jb5 | SCENE= }}
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|RELATEDENTRY=[[2c0v|2C0V]], [[2jb6|2JB6]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jb5 OCA], [http://www.ebi.ac.uk/pdbsum/2jb5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jb5 RCSB]</span>
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}}
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'''FAB FRAGMENT IN COMPLEX WITH SMALL MOLECULE HAPTEN, CRYSTAL FORM-1'''
'''FAB FRAGMENT IN COMPLEX WITH SMALL MOLECULE HAPTEN, CRYSTAL FORM-1'''
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[[Category: Malawski, G.]]
[[Category: Malawski, G.]]
[[Category: Schirner, M.]]
[[Category: Schirner, M.]]
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[[Category: antibody fragment]]
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[[Category: Antibody fragment]]
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[[Category: cdr]]
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[[Category: Cdr]]
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[[Category: diagnostic imaging]]
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[[Category: Diagnostic imaging]]
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[[Category: fab]]
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[[Category: Fab]]
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[[Category: fluorescent dye]]
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[[Category: Fluorescent dye]]
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[[Category: hucal]]
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[[Category: Hucal]]
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[[Category: immune system]]
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[[Category: Immune system]]
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[[Category: immunoglobulin domain]]
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[[Category: Immunoglobulin domain]]
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[[Category: tsc]]
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[[Category: Tsc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:38:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:55:42 2008''
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Revision as of 05:38, 4 May 2008

Image:2jb5.jpg

Template:STRUCTURE 2jb5

FAB FRAGMENT IN COMPLEX WITH SMALL MOLECULE HAPTEN, CRYSTAL FORM-1


Overview

Molecular interactions between near-IR fluorescent probes and specific antibodies may be exploited to generate novel smart probes for diagnostic imaging. Using a new phage display technology, we developed such antibody Fab fragments with subnanomolar binding affinity for tetrasulfocyanine, a near-IR in vivo imaging agent. Unexpectedly, some Fabs induced redshifts of the dye absorption peak of up to 44 nm. This is the largest shift reported for a biological system so far. Crystal structure determination and absorption spectroscopy in the crystal in combination with microcalorimetry and small-angle X-ray scattering in solution revealed that the redshift is triggered by formation of a Fab dimer, with tetrasulfocyanine being buried in a fully closed protein cavity within the dimer interface. The derived principle of shifting the absorption peak of a symmetric dye via packaging within a Fab dimer interface may be transferred to other diagnostic fluorophores, opening the way towards smart imaging probes that change their wavelength upon interaction with an antibody.

About this Structure

2JB5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded fab dimer interface., Hillig RC, Urlinger S, Fanghanel J, Brocks B, Haenel C, Stark Y, Sulzle D, Svergun DI, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K, J Mol Biol. 2008 Mar 14;377(1):206-19. Epub 2008 Jan 5. PMID:18241888 Page seeded by OCA on Sun May 4 08:38:11 2008

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OCA

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