3hck
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(New page: 200px<br /> <applet load="3hck" size="450" color="white" frame="true" align="right" spinBox="true" caption="3hck" /> '''NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK S...)
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Revision as of 21:42, 12 November 2007
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NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK SH2 DOMAIN, 20 STRUCTURES
Overview
The hematopoietic cellular kinase (Hck) is a member of the Src family of, non-receptor protein-tyrosine kinases and participates in signal, transduction events regulating the growth, differentiation and function of, phagocytes. The secondary structure of the SH2 domain for Hck was, determined for a 13C/15N-enriched sample using multi-dimensional NMR, spectroscopy. The secondary structure for the domain was determined from, chemical shift indices [1H alpha, 13C alpha and 13C'], sequential NOEs, [d(alphaN)(i, i+1) and d(NN)(i, i+1)], and 3J(alphaN) scalar coupling, constants. The Hck SH2 domain consists of two alpha-helices and seven, beta-strands. Complementary strands of beta-sheets were identified from, long-range NOEs using a novel 3D, 13C/15N-edited HMQC-NOESY-(HCACO)NH, experiment that correlated 1H alpha resonances between beta-strands. The, secondary structure for Hck SH2 is similar to that predicted from the, sequence alignment of the Src-family protein tyrosine kinases.
About this Structure
3HCK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Sequential assignment and secondary structure determination for the Src homology 2 domain of hematopoietic cellular kinase., Zhang W, Smithgall TE, Gmeiner WH, FEBS Lett. 1997 Apr 7;406(1-2):131-5. PMID:9109402
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