3htc
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(New page: 200px<br /> <applet load="3htc" size="450" color="white" frame="true" align="right" spinBox="true" caption="3htc, resolution 2.3Å" /> '''THE STRUCTURE OF A C...)
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Revision as of 21:42, 12 November 2007
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THE STRUCTURE OF A COMPLEX OF RECOMBINANT HIRUDIN AND HUMAN ALPHA-THROMBIN
Contents |
Overview
The crystallographic structure of a recombinant hirudin-thrombin complex, has been solved at 2.3 angstrom (A) resolution. Hirudin consists of an, NH2-terminal globular domain and a long (39 A) COOH-terminal extended, domain. Residues Ile1 to Tyr3 of hirudin form a parallel beta-strand with, Ser214 to Glu217 of thrombin with the nitrogen atom of Ile1 making a, hydrogen bond with Ser195 O gamma atom of the catalytic site, but the, specificity pocket of thrombin is not involved in the interaction. The, COOH-terminal segment makes numerous electrostatic interactions with an, anion-binding exosite of thrombin, whereas the last five residues are in a, helical loop that forms many hydrophobic contacts. In all, 27 of the 65, residues of hirudin have contacts less than 4.0 A with thrombin (10 ion, pairs and 23 hydrogen bonds). Such abundant interactions may account for, the high affinity and specificity of hirudin.
Disease
Known diseases associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]
About this Structure
3HTC is a Protein complex structure of sequences from Hirudinaria manillensis and Homo sapiens. Active as Thrombin, with EC number 3.4.21.5 Full crystallographic information is available from OCA.
Reference
The structure of a complex of recombinant hirudin and human alpha-thrombin., Rydel TJ, Ravichandran KG, Tulinsky A, Bode W, Huber R, Roitsch C, Fenton JW 2nd, Science. 1990 Jul 20;249(4966):277-80. PMID:2374926
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