2jd3
From Proteopedia
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'''PARR FROM PLASMID PB171''' | '''PARR FROM PLASMID PB171''' | ||
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[[Category: Lowe, J.]] | [[Category: Lowe, J.]] | ||
[[Category: Moller-Jensen, J.]] | [[Category: Moller-Jensen, J.]] | ||
| - | [[Category: | + | [[Category: Actin-like filament]] |
| - | [[Category: | + | [[Category: Dna binding]] |
| - | [[Category: | + | [[Category: Dna binding protein]] |
| - | [[Category: | + | [[Category: Parm]] |
| - | [[Category: | + | [[Category: Plasmid]] |
| - | [[Category: | + | [[Category: Plasmid segregation]] |
| - | [[Category: | + | [[Category: Rhh2]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:43:19 2008'' | |
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Revision as of 05:43, 4 May 2008
PARR FROM PLASMID PB171
Overview
Accurate DNA partition at cell division is vital to all living organisms. In bacteria, this process can involve partition loci, which are found on both chromosomes and plasmids. The initial step in Escherichia coli plasmid R1 partition involves the formation of a partition complex between the DNA-binding protein ParR and its cognate centromere site parC on the DNA. The partition complex is recognized by a second partition protein, the actin-like ATPase ParM, which forms filaments required for the active bidirectional movement of DNA replicates. Here, we present the 2.8 A crystal structure of ParR from E. coli plasmid pB171. ParR forms a tight dimer resembling a large family of dimeric ribbon-helix-helix (RHH)2 site-specific DNA-binding proteins. Crystallographic and electron microscopic data further indicate that ParR dimers assemble into a helix structure with DNA-binding sites facing outward. Genetic and biochemical experiments support a structural arrangement in which the centromere-like parC DNA is wrapped around a ParR protein scaffold. This structure holds implications for how ParM polymerization drives active DNA transport during plasmid partition.
About this Structure
2JD3 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural analysis of the ParR/parC plasmid partition complex., Moller-Jensen J, Ringgaard S, Mercogliano CP, Gerdes K, Lowe J, EMBO J. 2007 Oct 17;26(20):4413-22. Epub 2007 Sep 27. PMID:17898804 Page seeded by OCA on Sun May 4 08:43:19 2008
