2jdf

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[[Image:2jdf.jpg|left|200px]]
[[Image:2jdf.jpg|left|200px]]
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{{Structure
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|PDB= 2jdf |SIZE=350|CAPTION= <scene name='initialview01'>2jdf</scene>, resolution 1.7&Aring;
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The line below this paragraph, containing "STRUCTURE_2jdf", creates the "Structure Box" on the page.
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{{STRUCTURE_2jdf| PDB=2jdf | SCENE= }}
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|RELATEDENTRY=[[1leu|1LEU]], [[1myv|1MYV]], [[1myx|1MYX]], [[1myy|1MYY]], [[1mz1|1MZ1]], [[1mz2|1MZ2]], [[1mz3|1MZ3]], [[2jdg|2JDG]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jdf OCA], [http://www.ebi.ac.uk/pdbsum/2jdf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jdf RCSB]</span>
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}}
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'''HUMAN GAMMA-B CRYSTALLIN'''
'''HUMAN GAMMA-B CRYSTALLIN'''
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[[Category: Scheuermann, T.]]
[[Category: Scheuermann, T.]]
[[Category: Stubbs, M T.]]
[[Category: Stubbs, M T.]]
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[[Category: affilin]]
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[[Category: Affilin]]
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[[Category: artificial binding protein]]
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[[Category: Artificial binding protein]]
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[[Category: eye lens protein]]
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[[Category: Eye lens protein]]
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[[Category: gamma crystallin]]
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[[Category: Gamma crystallin]]
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[[Category: oxidation]]
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[[Category: Oxidation]]
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[[Category: phosphorylation]]
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[[Category: Phosphorylation]]
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[[Category: polymorphism]]
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[[Category: Polymorphism]]
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[[Category: structural protein]]
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[[Category: Structural protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:44:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:56:33 2008''
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Revision as of 05:44, 4 May 2008

Image:2jdf.jpg

Template:STRUCTURE 2jdf

HUMAN GAMMA-B CRYSTALLIN


Overview

The concept of novel binding proteins as an alternative to antibodies has undergone rapid development and is now ready for practical use in a wide range of applications. Alternative binding proteins, based on suitable scaffolds with desirable properties, are selected from combinatorial libraries in vitro. Here, we describe an approach using a beta-sheet of human gamma-B-crystallin to generate a universal binding site through randomization of eight solvent-exposed amino acid residues selected according to structural and sequence analyses. Specific variants, so-called Affilin, have been isolated from a phage display library against a variety of targets that differ considerably in size and structure. The isolated Affilin variants can be produced in Escherichia coli as soluble proteins and have a high level of thermodynamic stability. The crystal structures of the human wild-type gamma-B-crystallin and a selected Affilin variant have been determined to 1.7 A and 2.0 A resolution, respectively. Comparison of the two molecules indicates that the human gamma-B-crystallin tolerates amino acid exchanges with no major structural change. We conclude that the intrinsically stable and easily expressed gamma-B-crystallin provides a suitable framework for the generation of novel binding molecules.

About this Structure

2JDF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Affilin-novel binding molecules based on human gamma-B-crystallin, an all beta-sheet protein., Ebersbach H, Fiedler E, Scheuermann T, Fiedler M, Stubbs MT, Reimann C, Proetzel G, Rudolph R, Fiedler U, J Mol Biol. 2007 Sep 7;372(1):172-85. Epub 2007 Jun 22. PMID:17628592 Page seeded by OCA on Sun May 4 08:44:04 2008

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