2je3
From Proteopedia
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'''CYTOCHROME P460 FROM NITROSOMONAS EUROPAEA- PROBABLE PHYSIOLOGICAL FORM''' | '''CYTOCHROME P460 FROM NITROSOMONAS EUROPAEA- PROBABLE PHYSIOLOGICAL FORM''' | ||
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[[Category: Wilmot, C M.]] | [[Category: Wilmot, C M.]] | ||
[[Category: Yang, C.]] | [[Category: Yang, C.]] | ||
| - | [[Category: | + | [[Category: Cross-linked heme]] |
| - | [[Category: | + | [[Category: Cytochrome p460]] |
| - | [[Category: | + | [[Category: Heme p460]] |
| - | [[Category: | + | [[Category: Metal binding protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:45:56 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:45, 4 May 2008
CYTOCHROME P460 FROM NITROSOMONAS EUROPAEA- PROBABLE PHYSIOLOGICAL FORM
Overview
We have determined the 1.8 A X-ray crystal structure of a monoheme c-type cytochrome, cytochrome P460, from Nitrosomonas europea. The chromophore possesses unusual spectral properties analogous to those of the catalytic heme P460 of hydroxylamine oxidoreductase (HAO), the only known heme in biology to withdraw electrons from an iron-coordinated substrate. The analysis reveals a homodimeric structure and elucidates a new c-type cytochrome fold that is predominantly beta-sheet. In addition to the two cysteine thioether links to the porphyrin typical of c-type hemes, there is a third proteinaceous link involving a conserved lysine. The covalent bond is between the lysine side-chain nitrogen and the 13'-meso carbon of the heme, which, following cross-link formation, is sp3-hybridized, demonstrating the loss of conjugation at this position within the porphyrin. The structure has implications for the analogous tyrosine-heme meso carbon cross-link observed in HAO.
About this Structure
2JE3 is a Single protein structure of sequence from Nitrosomonas europaea. Full crystallographic information is available from OCA.
Reference
The crystal structure of cytochrome P460 of Nitrosomonas europaea reveals a novel cytochrome fold and heme-protein cross-link., Pearson AR, Elmore BO, Yang C, Ferrara JD, Hooper AB, Wilmot CM, Biochemistry. 2007 Jul 17;46(28):8340-9. Epub 2007 Jun 21. PMID:17583915 Page seeded by OCA on Sun May 4 08:45:56 2008
