This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2jm3
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2jm3.jpg|left|200px]] | [[Image:2jm3.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2jm3", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2jm3| PDB=2jm3 | SCENE= }} | |
| - | | | + | |
| - | | | + | |
| - | }} | + | |
'''Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)''' | '''Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)''' | ||
| Line 29: | Line 26: | ||
[[Category: Mackay, J P.]] | [[Category: Mackay, J P.]] | ||
[[Category: Nicholas, H R.]] | [[Category: Nicholas, H R.]] | ||
| - | [[Category: | + | [[Category: Domain]] |
| - | [[Category: | + | [[Category: Protein]] |
| - | [[Category: | + | [[Category: Zinc finger]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:01:00 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:01, 4 May 2008
Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)
Overview
The THAP (Thanatos-associated protein) domain is a recently discovered zinc-binding domain found in proteins involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. It contains a single zinc atom ligated by cysteine and histidine residues within a Cys-X(2-4)-Cys-X(35-53)-Cys-X(2)-His consensus. We have determined the NMR solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP) and show that it adopts a fold containing a treble clef motif, bearing similarity to the zinc finger-associated domain (ZAD) from Drosophila Grauzone. The CtBP THAP domain contains a large, positively charged surface patch and we demonstrate that this domain can bind to double-stranded DNA in an electrophoretic mobility-shift assay. These data, together with existing reports, indicate that THAP domains might exhibit a functional diversity similar to that observed for classical and GATA-type zinc fingers.
About this Structure
2JM3 is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP)., Liew CK, Crossley M, Mackay JP, Nicholas HR, J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978 Page seeded by OCA on Sun May 4 09:01:00 2008
