2jmc
From Proteopedia
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[[Image:2jmc.gif|left|200px]] | [[Image:2jmc.gif|left|200px]] | ||
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'''Chimer between Spc-SH3 and P41''' | '''Chimer between Spc-SH3 and P41''' | ||
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[[Category: Martinez, J C.]] | [[Category: Martinez, J C.]] | ||
[[Category: Nuland, N A.J van.]] | [[Category: Nuland, N A.J van.]] | ||
| - | [[Category: | + | [[Category: Chimer]] |
| - | [[Category: | + | [[Category: P41]] |
| - | [[Category: | + | [[Category: Spc-sh3]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:01:36 2008'' | |
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Revision as of 06:01, 4 May 2008
Chimer between Spc-SH3 and P41
Overview
Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design.
About this Structure
2JMC is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex., Candel AM, Conejero-Lara F, Martinez JC, van Nuland NA, Bruix M, FEBS Lett. 2007 Feb 20;581(4):687-92. Epub 2007 Jan 22. PMID:17275816 Page seeded by OCA on Sun May 4 09:01:36 2008
