7fab
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(New page: 200px<br /> <applet load="7fab" size="450" color="white" frame="true" align="right" spinBox="true" caption="7fab, resolution 2.0Å" /> '''CRYSTAL STRUCTURE OF...)
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Revision as of 21:46, 12 November 2007
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CRYSTAL STRUCTURE OF HUMAN IMMUNOGLOBULIN FRAGMENT FAB NEW REFINED AT 2.0 ANGSTROMS RESOLUTION
Overview
The three-dimensional structure of the human immunoglobulin fragment Fab, New (IgG1, lambda) has been refined to a crystallographic R-factor of, 16.9% to 2 A resolution. Rms deviations of the final model from ideal, geometry are 0.014 A for bond distances and 3.03 degrees for bond angles., Refinement was based on a new X-ray data set including 28,301 reflections, with F > 2.5 sigma(F) from 6.0 to 2.0 A resolution. The starting model for, the refinement procedure reported here is from the Brookhaven Protein Data, Bank entry 3FAB (rev. 1981). Differences between the initial and final, models include modified polypeptide-chain folding in the third, complementarity-determining region (CDR3) and the third framework region, (FR3) of VH and in some exposed loops of CL and CH1. Amino acid sequence, changes were determined at a number of positions by inspection of, difference electron density maps. The incorporation of amino acid sequence, changes results in an improved VH framework model for the "humanization", of monoclonal antibodies.
About this Structure
7FAB is a Protein complex structure of sequences from Homo sapiens. This structure superseeds the now removed PDB entries 3FAB, 2FAB and 1FAB. Full crystallographic information is available from OCA.
Reference
Crystal structure of human immunoglobulin fragment Fab New refined at 2.0 A resolution., Saul FA, Poljak RJ, Proteins. 1992 Nov;14(3):363-71. PMID:1438175
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