2jo9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2jo9.gif|left|200px]]
[[Image:2jo9.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2jo9 |SIZE=350|CAPTION= <scene name='initialview01'>2jo9</scene>
+
The line below this paragraph, containing "STRUCTURE_2jo9", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND=
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= Itch ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2jo9| PDB=2jo9 | SCENE= }}
-
|RELATEDENTRY=[[2joc|2JOC]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jo9 OCA], [http://www.ebi.ac.uk/pdbsum/2jo9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jo9 RCSB]</span>
+
-
}}
+
'''Mouse Itch 3rd WW domain complex with the Epstein-Barr virus latent membrane protein 2A derived peptide EEPPPPYED'''
'''Mouse Itch 3rd WW domain complex with the Epstein-Barr virus latent membrane protein 2A derived peptide EEPPPPYED'''
Line 33: Line 30:
[[Category: Shaw, A Z.]]
[[Category: Shaw, A Z.]]
[[Category: Yraola, F.]]
[[Category: Yraola, F.]]
-
[[Category: complex]]
+
[[Category: Complex]]
-
[[Category: epstein-barr virus]]
+
[[Category: Epstein-barr virus]]
-
[[Category: itch]]
+
[[Category: Itch]]
-
[[Category: lmp2a]]
+
[[Category: Lmp2a]]
-
[[Category: ww]]
+
[[Category: Ww]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:06:07 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:59:58 2008''
+

Revision as of 06:06, 4 May 2008

Image:2jo9.gif

Template:STRUCTURE 2jo9

Mouse Itch 3rd WW domain complex with the Epstein-Barr virus latent membrane protein 2A derived peptide EEPPPPYED


Overview

In this work, we study the role of phosphorylation as a regulatory mechanism for the interaction between the E3 ubiquitin ligase ItchWW3 domain and two PPxY motifs of one of its targets, the Epstein-Barr virus latent membrane protein 2A. Whereas ligand phosphorylation only diminishes binding, domain phosphorylation at residue T30 abrogates it. We show that two ItchWW domains can be phosphorylated at this position, using CK2 and PKA kinases and/or with stimulated T lymphocyte lysates. To better understand the regulation process, we determined the NMR structures of the ItchWW3-PPxY complex and of the phosphoT30-ItchWW3 variant. The peptide binds the domain using both XP and tyrosine grooves. A hydrogen bond from T30 to the ligand is also detected. This hydrogen-bond formation is precluded in the variant, explaining the inhibition upon phosphorylation. Our results suggest that phosphorylation at position 30 in ItchWW domains can be a mechanism to inhibit target recognition in vivo.

About this Structure

2JO9 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands., Morales B, Ramirez-Espain X, Shaw AZ, Martin-Malpartida P, Yraola F, Sanchez-Tillo E, Farrera C, Celada A, Royo M, Macias MJ, Structure. 2007 Apr;15(4):473-83. PMID:17437719 Page seeded by OCA on Sun May 4 09:06:07 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jo9"
Personal tools