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2jpp
From Proteopedia
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[[Image:2jpp.gif|left|200px]] | [[Image:2jpp.gif|left|200px]] | ||
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'''Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA''' | '''Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA''' | ||
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[[Category: Oberstrass, F C.]] | [[Category: Oberstrass, F C.]] | ||
[[Category: Schubert, M.]] | [[Category: Schubert, M.]] | ||
| - | [[Category: | + | [[Category: Csra]] |
| - | [[Category: | + | [[Category: Protein/rna]] |
| - | [[Category: | + | [[Category: Rna recognition]] |
| - | [[Category: | + | [[Category: Rsma]] |
| - | [[Category: | + | [[Category: Shine-dalgarno]] |
| - | [[Category: | + | [[Category: Translation/rna complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:09:37 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:09, 4 May 2008
Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA
Overview
Proteins of the RsmA/CsrA family are global translational regulators in many bacterial species. We have determined the solution structure of a complex formed between the RsmE protein, a member of this family from Pseudomonas fluorescens, and a target RNA encompassing the ribosome-binding site of the hcnA gene. The RsmE homodimer with its two RNA-binding sites makes optimal contact with an 5'-A/UCANGGANGU/A-3' sequence in the mRNA. When tightly gripped by RsmE, the ANGGAN core folds into a loop, favoring the formation of a 3-base-pair stem by flanking nucleotides. We validated these findings by in vivo and in vitro mutational analyses. The structure of the complex explains well how, by sequestering the Shine-Dalgarno sequence, the RsmA/CsrA proteins repress translation.
About this Structure
2JPP is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.
Reference
Molecular basis of messenger RNA recognition by the specific bacterial repressing clamp RsmA/CsrA., Schubert M, Lapouge K, Duss O, Oberstrass FC, Jelesarov I, Haas D, Allain FH, Nat Struct Mol Biol. 2007 Sep;14(9):807-13. Epub 2007 Aug 19. PMID:17704818 Page seeded by OCA on Sun May 4 09:09:37 2008
