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2jv1

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[[Image:2jv1.jpg|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_2jv1", creates the "Structure Box" on the page.
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|GENE= INS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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{{STRUCTURE_2jv1| PDB=2jv1 | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jv1 OCA], [http://www.ebi.ac.uk/pdbsum/2jv1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jv1 RCSB]</span>
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'''NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures'''
'''NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures'''
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[[Category: Kozerski, L.]]
[[Category: Kozerski, L.]]
[[Category: 35% cd3cn]]
[[Category: 35% cd3cn]]
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[[Category: carbohydrate metabolism]]
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[[Category: Carbohydrate metabolism]]
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[[Category: cleavage on pair of basic residue]]
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[[Category: Cleavage on pair of basic residue]]
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[[Category: diabetes mellitus]]
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[[Category: Diabetes mellitus]]
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[[Category: disease mutation]]
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[[Category: Disease mutation]]
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[[Category: glucose metabolism]]
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[[Category: Glucose metabolism]]
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[[Category: hormone]]
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[[Category: Hormone]]
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[[Category: human insulin]]
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[[Category: Human insulin]]
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[[Category: monomer]]
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[[Category: Monomer]]
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[[Category: nmr]]
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[[Category: Nmr]]
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[[Category: pharmaceutical]]
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[[Category: Pharmaceutical]]
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[[Category: secreted]]
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[[Category: Secreted]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:19:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:01:54 2008''
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Revision as of 06:19, 4 May 2008

Image:2jv1.jpg

Template:STRUCTURE 2jv1

NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures


Contents

Overview

Here we present evidence that in water/acetonitrile solvent detailed structural and dynamic information can be obtained for important proteins that are naturally present as oligomers under native conditions. An NMR-derived human insulin monomer structure in H(2)O/CD(3)CN, 65/35 vol%, pH 3.6 is presented and compared with the available X-ray structure of a monomer that forms part of a hexamer (Acta Crystallogr. 2003 Sec. D59, 474) and with NMR structures in water and organic cosolvent. Detailed analysis using PFGSE NMR, temperature-dependent NMR, dilution experiments and CSI proves that the structure is monomeric in the concentration and temperature ranges 0.1-3 mM and 10-30 degrees C, respectively. The presence of long-range interstrand NOEs, as found in the crystal structure of the monomer, provides the evidence for conservation of the tertiary structure. Starting from structures calculated by the program CYANA, two different molecular dynamics simulated annealing refinement protocols were applied, either using the program AMBER in vacuum (AMBER_VC), or including a generalized Born solvent model (AMBER_GB).

Disease

Known disease associated with this structure: Diabetes mellitus, rare form OMIM:[176730], Hyperproinsulinemia, familial OMIM:[176730], MODY, one form OMIM:[176730]

About this Structure

2JV1 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of human insulin monomer in water/acetonitrile solution., Bocian W, Sitkowski J, Bednarek E, Tarnowska A, Kawecki R, Kozerski L, J Biomol NMR. 2008 Jan;40(1):55-64. Epub 2007 Nov 27. PMID:18040865 Page seeded by OCA on Sun May 4 09:19:13 2008

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