2jvb

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[[Image:2jvb.jpg|left|200px]]
[[Image:2jvb.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2jvb |SIZE=350|CAPTION= <scene name='initialview01'>2jvb</scene>
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The line below this paragraph, containing "STRUCTURE_2jvb", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|GENE= DCP2, PSU1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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|DOMAIN=
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{{STRUCTURE_2jvb| PDB=2jvb | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jvb OCA], [http://www.ebi.ac.uk/pdbsum/2jvb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jvb RCSB]</span>
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'''Solution Structure of Catalytic Domain of yDcp2'''
'''Solution Structure of Catalytic Domain of yDcp2'''
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[[Category: Deshmukh, M.]]
[[Category: Deshmukh, M.]]
[[Category: Gross, J.]]
[[Category: Gross, J.]]
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[[Category: cytoplasm]]
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[[Category: Cytoplasm]]
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[[Category: dcp2]]
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[[Category: Dcp2]]
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[[Category: decapping]]
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[[Category: Decapping]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: manganese]]
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[[Category: Manganese]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: mrna decay]]
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[[Category: Mrna decay]]
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[[Category: mrna processing]]
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[[Category: Mrna processing]]
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[[Category: nonsense-mediated mrna decay]]
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[[Category: Nonsense-mediated mrna decay]]
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[[Category: nucleus]]
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[[Category: Nucleus]]
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[[Category: phosphorylation]]
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[[Category: Phosphorylation]]
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[[Category: rna-binding]]
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[[Category: Rna-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:19:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:02:01 2008''
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Revision as of 06:19, 4 May 2008

Image:2jvb.jpg

Template:STRUCTURE 2jvb

Solution Structure of Catalytic Domain of yDcp2


Overview

Cap hydrolysis by Dcp2 is a critical step in several eukaryotic mRNA decay pathways. Processing requires access to cap-proximal nucleotides and the coordinated assembly of a decapping mRNP, but the mechanism of substrate recognition and regulation by protein interactions have remained elusive. Using NMR spectroscopy and kinetic analyses, we show that yeast Dcp2 resolves interactions with the cap and RNA body using a bipartite surface that forms a channel intersecting the catalytic and regulatory Dcp1-binding domains. The interaction with cap is weak but specific and requires binding of the RNA body to a dynamic interface. The catalytic step is stimulated by Dcp1 and its interaction domain, likely through a substrate-induced conformational change. Thus, activation of the decapping mRNP is restricted by access to 5'-proximal nucleotides, a feature that could act as a checkpoint in mRNA metabolism.

About this Structure

2JVB is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

mRNA Decapping Is Promoted by an RNA-Binding Channel in Dcp2., Deshmukh MV, Jones BN, Quang-Dang DU, Flinders J, Floor SN, Kim C, Jemielity J, Kalek M, Darzynkiewicz E, Gross JD, Mol Cell. 2008 Feb 15;29(3):324-36. PMID:18280238 Page seeded by OCA on Sun May 4 09:19:41 2008

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