2jy7

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[[Image:2jy7.jpg|left|200px]]
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{{Structure
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|PDB= 2jy7 |SIZE=350|CAPTION= <scene name='initialview01'>2jy7</scene>
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|GENE= SQSTM1, ORCA, OSIL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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{{STRUCTURE_2jy7| PDB=2jy7 | SCENE= }}
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|RELATEDENTRY=[[2jy8|2JY8]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jy7 OCA], [http://www.ebi.ac.uk/pdbsum/2jy7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jy7 RCSB]</span>
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'''NMR structure of the ubiquitin associated (UBA) domain of p62 (SQSTM1). RDC refined'''
'''NMR structure of the ubiquitin associated (UBA) domain of p62 (SQSTM1). RDC refined'''
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[[Category: Long, J E.]]
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[[Category: Ubiquitin associated domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:23:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:02:32 2008''
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Revision as of 06:23, 4 May 2008

Image:2jy7.jpg

Template:STRUCTURE 2jy7

NMR structure of the ubiquitin associated (UBA) domain of p62 (SQSTM1). RDC refined


Overview

The p62 protein functions as a scaffold in signaling pathways that lead to activation of NF-kappaB and is an important regulator of osteoclastogenesis. Mutations affecting the receptor activator of NF-kappaB signaling axis can result in human skeletal disorders, including those identified in the C-terminal ubiquitin-associated (UBA) domain of p62 in patients with Paget disease of bone. These observations suggest that the disease may involve a common mechanism related to alterations in the ubiquitin-binding properties of p62. The structural basis for ubiquitin recognition by the UBA domain of p62 has been investigated using NMR and reveals a novel binding mechanism involving a slow exchange structural reorganization of the UBA domain to a "bound" non-canonical UBA conformation that is not significantly populated in the absence of ubiquitin. The repacking of the three-helix bundle generates a binding surface localized around the conserved Xaa-Gly-Phe-Xaa loop that appears to optimize both hydrophobic and electrostatic surface complementarity with ubiquitin. NMR titration analysis shows that the p62-UBA binds to Lys(48)-linked di-ubiquitin with approximately 4-fold lower affinity than to mono-ubiquitin, suggesting preferential binding of the p62-UBA to single ubiquitin units, consistent with the apparent in vivo preference of the p62 protein for Lys(63)-linked polyubiquitin chains (which adopt a more open and extended structure). The conformational switch observed on binding may represent a novel mechanism that underlies specificity in regulating signalinduced protein recognition events.

About this Structure

2JY7 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Ubiquitin Recognition by the Ubiquitin-associated Domain of p62 Involves a Novel Conformational Switch., Long J, Gallagher TR, Cavey JR, Sheppard PW, Ralston SH, Layfield R, Searle MS, J Biol Chem. 2008 Feb 29;283(9):5427-5440. Epub 2007 Dec 14. PMID:18083707 Page seeded by OCA on Sun May 4 09:23:29 2008

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