2kau

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2kau.jpg|left|200px]]
[[Image:2kau.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2kau |SIZE=350|CAPTION= <scene name='initialview01'>2kau</scene>, resolution 2.0&Aring;
+
The line below this paragraph, containing "STRUCTURE_2kau", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= UREC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28451 Klebsiella aerogenes]), UREB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28451 Klebsiella aerogenes]), UREA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28451 Klebsiella aerogenes])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2kau| PDB=2kau | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kau OCA], [http://www.ebi.ac.uk/pdbsum/2kau PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2kau RCSB]</span>
+
-
}}
+
'''THE CRYSTAL STRUCTURE OF UREASE FROM KLEBSIELLA AEROGENES AT 2.2 ANGSTROMS RESOLUTION'''
'''THE CRYSTAL STRUCTURE OF UREASE FROM KLEBSIELLA AEROGENES AT 2.2 ANGSTROMS RESOLUTION'''
Line 30: Line 27:
[[Category: Jabri, E.]]
[[Category: Jabri, E.]]
[[Category: Karplus, P A.]]
[[Category: Karplus, P A.]]
-
[[Category: nickel metalloenzyme]]
+
[[Category: Nickel metalloenzyme]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:28:49 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:03:06 2008''
+

Revision as of 06:28, 4 May 2008

Image:2kau.jpg

Template:STRUCTURE 2kau

THE CRYSTAL STRUCTURE OF UREASE FROM KLEBSIELLA AEROGENES AT 2.2 ANGSTROMS RESOLUTION


Overview

The crystal structure of urease from Klebsiella aerogenes has been determined at 2.2 A resolution and refined to an R factor of 18.2 percent. The enzyme contains four structural domains: three with novel folds playing structural roles, and an (alpha beta)8 barrel domain, which contains the bi-nickel center. The two active site nickels are 3.5 A apart. One nickel ion is coordinated by three ligands (with low occupancy of a fourth ligand) and the second is coordinated by five ligands. A carbamylated lysine provides an oxygen ligand to each nickel, explaining why carbon dioxide is required for the activation of urease apoenzyme. The structure is compatible with a catalytic mechanism whereby urea ligates Ni-1 to complete its tetrahedral coordination and a hydroxide ligand of Ni-2 attacks the carbonyl carbon. A surprisingly high structural similarity between the urease catalytic domain and that of the zinc-dependent adenosine deaminase reveals a remarkable example of active site divergence.

About this Structure

2KAU is a Protein complex structure of sequences from Klebsiella aerogenes. Full crystallographic information is available from OCA.

Reference

The crystal structure of urease from Klebsiella aerogenes., Jabri E, Carr MB, Hausinger RP, Karplus PA, Science. 1995 May 19;268(5213):998-1004. PMID:7754395 Page seeded by OCA on Sun May 4 09:28:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kau"
Personal tools