2ltn

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[[Image:2ltn.jpg|left|200px]]
[[Image:2ltn.jpg|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_2ltn", creates the "Structure Box" on the page.
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{{STRUCTURE_2ltn| PDB=2ltn | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ltn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltn OCA], [http://www.ebi.ac.uk/pdbsum/2ltn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ltn RCSB]</span>
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'''DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)'''
'''DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)'''
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[[Category: Phillips, S R.]]
[[Category: Phillips, S R.]]
[[Category: Suddath, F L.]]
[[Category: Suddath, F L.]]
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[[Category: lectin]]
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[[Category: Lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:31:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:03:37 2008''
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Revision as of 06:31, 4 May 2008

Image:2ltn.jpg

Template:STRUCTURE 2ltn

DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)


Overview

The propeptide form of the lectin from the garden pea (Pisum sativum agglutinin) has been expressed in Escherichia coli by attaching its cDNA to an inducible promoter. By a number of criteria, including the ability to form dimers, hemagglutination titer, Western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. Preliminary crystallization experiments using the recombinant propeptide lectin yield crystals in space group P2(1)2(1)2(1) with a = 64.8 A, b = 73.8 A, and c = 109.0 A (1 A = 0.1 nm) that diffract to 2.8-A resolution. This unit cell size is quite similar to the unit cell determined for native pea lectin, suggesting that the overall structure of the recombinant prolectin is virtually identical.

About this Structure

2LTN is a Protein complex structure of sequences from Pisum sativum. Full crystallographic information is available from OCA.

Reference

Design, expression, and crystallization of recombinant lectin from the garden pea (Pisum sativum)., Prasthofer T, Phillips SR, Suddath FL, Engler JA, J Biol Chem. 1989 Apr 25;264(12):6793-6. PMID:2708344 Page seeded by OCA on Sun May 4 09:31:08 2008

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