2mlp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2mlp.jpg|left|200px]]
[[Image:2mlp.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2mlp |SIZE=350|CAPTION= <scene name='initialview01'>2mlp</scene>
+
The line below this paragraph, containing "STRUCTURE_2mlp", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2mlp| PDB=2mlp | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlp OCA], [http://www.ebi.ac.uk/pdbsum/2mlp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2mlp RCSB]</span>
+
-
}}
+
'''MICROCIN LEADER PEPTIDE FROM E. COLI, NMR, 25 STRUCTURES'''
'''MICROCIN LEADER PEPTIDE FROM E. COLI, NMR, 25 STRUCTURES'''
Line 29: Line 26:
[[Category: Roy, R Sinha.]]
[[Category: Roy, R Sinha.]]
[[Category: Walsh, C T.]]
[[Category: Walsh, C T.]]
-
[[Category: antibiotic]]
+
[[Category: Antibiotic]]
-
[[Category: leader peptide]]
+
[[Category: Leader peptide]]
-
[[Category: propeptide]]
+
[[Category: Propeptide]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:34:00 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:04:15 2008''
+

Revision as of 06:34, 4 May 2008

Image:2mlp.jpg

Template:STRUCTURE 2mlp

MICROCIN LEADER PEPTIDE FROM E. COLI, NMR, 25 STRUCTURES


Overview

BACKGROUND: The peptide antibiotic microcin B17 (MccB17) contains oxazole and thiazole heterocycles formed by the post-translational modification of four cysteine and four serine residues. An amino-terminal propeptide targets the 69 amino acid precursor of MccB17 (preproMccB17) to the heterocyclization enzyme MccB17 synthetase. The mode of synthetase recognition has been unclear, because there has been limited structural information available on the MccB17 propeptide to date. RESULTS: The solution structure of the MccB17 propeptide (McbA1-26), determined using nuclear magnetic resonance, reveals that McbA1-26 is an amphipathic alpha helix. Mutational analysis of 13 propeptide residues showed that Phe8 and Leu12 are essential residues for MccB17 synthetase recognition. A domain of the propeptide was putatively identified as the region that interacts with the synthetase. CONCLUSIONS: MccB17 synthetase recognizes key hydrophobic residues within a helical propeptide, allowing the selective heterocyclization of downstream cysteine and serine residues in preproMccB17. The determination of the solution structure of the propeptide should facilitate the investigation of other functions of the propeptide, including a potential role in antibiotic secretion.

About this Structure

2MLP is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Role of the microcin B17 propeptide in substrate recognition: solution structure and mutational analysis of McbA1-26., Roy RS, Kim S, Baleja JD, Walsh CT, Chem Biol. 1998 Apr;5(4):217-28. PMID:9545435 Page seeded by OCA on Sun May 4 09:34:00 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mlp"
Personal tools