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2neo
From Proteopedia
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'''SOLUTION NMR STRUCTURE OF A TWO-BASE DNA BULGE COMPLEXED WITH AN ENEDIYNE CLEAVING ANALOG, 11 STRUCTURES''' | '''SOLUTION NMR STRUCTURE OF A TWO-BASE DNA BULGE COMPLEXED WITH AN ENEDIYNE CLEAVING ANALOG, 11 STRUCTURES''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1neo 1neo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NEO OCA]. | |
==Reference== | ==Reference== | ||
Solution structure of a two-base DNA bulge complexed with an enediyne cleaving analog., Stassinopoulos A, Ji J, Gao X, Goldberg IH, Science. 1996 Jun 28;272(5270):1943-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8658168 8658168] | Solution structure of a two-base DNA bulge complexed with an enediyne cleaving analog., Stassinopoulos A, Ji J, Gao X, Goldberg IH, Science. 1996 Jun 28;272(5270):1943-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8658168 8658168] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Gao, X.]] | [[Category: Gao, X.]] | ||
[[Category: Goldberg, I H.]] | [[Category: Goldberg, I H.]] | ||
[[Category: Ji, J.]] | [[Category: Ji, J.]] | ||
[[Category: Stassinopoulos, A.]] | [[Category: Stassinopoulos, A.]] | ||
| - | [[Category: | + | [[Category: Bulge binding ligand]] |
| - | [[Category: | + | [[Category: Bulge dna]] |
| - | [[Category: | + | [[Category: Carbohydrate]] |
| - | [[Category: | + | [[Category: Deoxyribonucleic acid]] |
| - | [[Category: | + | [[Category: Dna]] |
| - | [[Category: | + | [[Category: Enediyne antibiotics antitumor agent]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:35:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:35, 4 May 2008
SOLUTION NMR STRUCTURE OF A TWO-BASE DNA BULGE COMPLEXED WITH AN ENEDIYNE CLEAVING ANALOG, 11 STRUCTURES
Overview
Nucleic acid bulges have been implicated in a number of biological processes and are specific cleavage targets for the enediyne antitumor antibiotic neocarzinostatin chromophore in a base-catalyzed, radical-mediated reaction. The solution structure of the complex between an analog of the bulge-specific cleaving species and an oligodeoxynucleotide containing a two-base bulge was elucidated by nuclear magnetic resonance. An unusual binding mode involves major groove recognition by the drug carbohydrate unit and tight fitting of the wedge-shaped drug in the triangular prism pocket formed by the two looped-out bulge bases and the neighboring base pairs. The two drug rings mimic helical DNA bases, complementing the bent DNA structure. The putative abstracting drug radical is 2.2 +/- 0.1 angstroms from the pro-S H5' of the target bulge nucleotide. This structure clarifies the mechanism of bulge recognition and cleavage by a drug and provides insight into the design of bulge-specific nucleic acid binding molecules.
About this Structure
This structure supersedes the now removed PDB entry 1neo. Full crystallographic information is available from OCA.
Reference
Solution structure of a two-base DNA bulge complexed with an enediyne cleaving analog., Stassinopoulos A, Ji J, Gao X, Goldberg IH, Science. 1996 Jun 28;272(5270):1943-6. PMID:8658168 Page seeded by OCA on Sun May 4 09:35:49 2008
