2ngr

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[[Image:2ngr.jpg|left|200px]]
[[Image:2ngr.jpg|left|200px]]
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{{Structure
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|PDB= 2ngr |SIZE=350|CAPTION= <scene name='initialview01'>2ngr</scene>, resolution 1.9&Aring;
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The line below this paragraph, containing "STRUCTURE_2ngr", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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{{STRUCTURE_2ngr| PDB=2ngr | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ngr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ngr OCA], [http://www.ebi.ac.uk/pdbsum/2ngr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ngr RCSB]</span>
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'''TRANSITION STATE COMPLEX FOR GTP HYDROLYSIS BY CDC42: COMPARISONS OF THE HIGH RESOLUTION STRUCTURES FOR CDC42 BOUND TO THE ACTIVE AND CATALYTICALLY COMPROMISED FORMS OF THE CDC42-GAP.'''
'''TRANSITION STATE COMPLEX FOR GTP HYDROLYSIS BY CDC42: COMPARISONS OF THE HIGH RESOLUTION STRUCTURES FOR CDC42 BOUND TO THE ACTIVE AND CATALYTICALLY COMPROMISED FORMS OF THE CDC42-GAP.'''
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[[Category: Hoffman, G.]]
[[Category: Hoffman, G.]]
[[Category: Nassar, N.]]
[[Category: Nassar, N.]]
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[[Category: alf3.]]
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[[Category: Alf3.]]
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[[Category: cdc42]]
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[[Category: Cdc42]]
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[[Category: g-protein]]
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[[Category: G-protein]]
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[[Category: gap]]
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[[Category: Gap]]
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[[Category: transition state]]
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[[Category: Transition state]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:36:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:04:46 2008''
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Revision as of 06:36, 4 May 2008

Image:2ngr.jpg

Template:STRUCTURE 2ngr

TRANSITION STATE COMPLEX FOR GTP HYDROLYSIS BY CDC42: COMPARISONS OF THE HIGH RESOLUTION STRUCTURES FOR CDC42 BOUND TO THE ACTIVE AND CATALYTICALLY COMPROMISED FORMS OF THE CDC42-GAP.


Overview

The Rho-related small GTP-binding protein Cdc42 has a low intrinsic GTPase activity that is significantly enhanced by its specific GTPase-activating protein, Cdc42GAP. In this report, we present the tertiary structure for the aluminum fluoride-promoted complex between Cdc42 and a catalytically active domain of Cdc42GAP as well as the complex between Cdc42 and the catalytically compromised Cdc42GAP(R305A) mutant. These structures, which mimic the transition state for the GTP hydrolytic reaction, show the presence of an AIF3 molecule, as was seen for the corresponding Ras-p120RasGAP complex, but in contrast to what has been reported for the Rho-Cdc42GAP complex or for heterotrimeric G protein alpha subunits, where AIF4- was observed. The Cdc42GAP stabilizes both the switch I and switch II domains of Cdc42 and contributes a highly conserved arginine (Arg 305) to the active site. Comparison of the structures for the wild type and mutant Cdc42GAP complexes provides important insights into the GAP-catalyzed GTP hydrolytic reaction.

About this Structure

2NGR is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structures of Cdc42 bound to the active and catalytically compromised forms of Cdc42GAP., Nassar N, Hoffman GR, Manor D, Clardy JC, Cerione RA, Nat Struct Biol. 1998 Dec;5(12):1047-52. PMID:9846874 Page seeded by OCA on Sun May 4 09:36:02 2008

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