2noo
From Proteopedia
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'''Crystal Structure of Mutant NikA''' | '''Crystal Structure of Mutant NikA''' | ||
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[[Category: Shimada, I.]] | [[Category: Shimada, I.]] | ||
[[Category: Tame, J R.H.]] | [[Category: Tame, J R.H.]] | ||
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| - | [[Category: | + | [[Category: Nickel-bound]] |
| - | [[Category: | + | [[Category: Periplasm]] |
| - | [[Category: | + | [[Category: Transport]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:42:47 2008'' | |
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Revision as of 06:42, 4 May 2008
Crystal Structure of Mutant NikA
Overview
Intracellular nickel is required by Escherichia coli as a cofactor for a number of enzymes and is necessary for anaerobic respiration. However, high concentrations of nickel are toxic, so both import and export systems have evolved to control the cellular level of the metal. The nik operon in E. coli encodes a nickel-uptake system that includes the periplasmic nickel-binding protein NikA. The crystal structures of wild-type NikA both bound to nickel and in the apo form have been solved previously. The liganded structure appeared to show an unusual interaction between the nickel and the protein in which no direct bonds are formed. The highly unusual nickel coordination suggested by the crystal structure contrasted strongly with earlier X-ray spectroscopic studies. The known nickel-binding site has been probed by extensive mutagenesis and isothermal titration calorimetry and it has been found that even large numbers of disruptive mutations appear to have little effect on the nickel affinity. The crystal structure of a binding-site mutant with nickel bound has been solved and it is found that nickel is bound to two histidine residues at a position distant from the previously characterized binding site. This novel site immediately resolves the conflict between the crystal structures and other biophysical analyses. The physiological relevance of the two binding sites is discussed.
About this Structure
2NOO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Nickel binding to NikA: an additional binding site reconciles spectroscopy, calorimetry and crystallography., Addy C, Ohara M, Kawai F, Kidera A, Ikeguchi M, Fuchigami S, Osawa M, Shimada I, Park SY, Tame JR, Heddle JG, Acta Crystallogr D Biol Crystallogr. 2007 Feb;63(Pt 2):221-9. Epub 2007, Jan 16. PMID:17242515 Page seeded by OCA on Sun May 4 09:42:47 2008
