2npf

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[[Image:2npf.gif|left|200px]]
[[Image:2npf.gif|left|200px]]
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{{Structure
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|PDB= 2npf |SIZE=350|CAPTION= <scene name='initialview01'>2npf</scene>, resolution 2.9&Aring;
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The line below this paragraph, containing "STRUCTURE_2npf", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MOU:(1S,4R,5R,9S,11S)-2-({[(2S,5R,6R,7R,9S,10R)-2-(7-CARBOXYHEPTYL)-6-HYDROXY-10-METHOXY-9-METHYL-3-OXO-1,4,8-TRIOXASPIRO[4.5]DEC-7-YL]OXY}METHYL)-9-FORMYL-13-ISOPROPYL-5-METHYLTETRACYCLO[7.4.0.02,11.04.8]TRIDEC-12-ENE-1-CARBOXYLIC+ACID'>MOU</scene>
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{{STRUCTURE_2npf| PDB=2npf | SCENE= }}
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|RELATEDENTRY=[[1n0u|1n0u]], [[2e1r|2E1R]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npf OCA], [http://www.ebi.ac.uk/pdbsum/2npf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2npf RCSB]</span>
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'''Structure of eEF2 in complex with moriniafungin'''
'''Structure of eEF2 in complex with moriniafungin'''
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[[Category: Mosley, R T.]]
[[Category: Mosley, R T.]]
[[Category: Soe, R.]]
[[Category: Soe, R.]]
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[[Category: g-protein]]
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[[Category: G-protein]]
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[[Category: protein-inhibitor complex]]
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[[Category: Protein-inhibitor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:44:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:06:23 2008''
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Revision as of 06:44, 4 May 2008

Image:2npf.gif

Template:STRUCTURE 2npf

Structure of eEF2 in complex with moriniafungin


Overview

The sordarins are fungal specific inhibitors of the translation factor eEF2, which catalyzes the translocation of tRNA and mRNA after peptide bond formation. We have determined the crystal structures of eEF2 in complex with two novel sordarin derivatives. In both structures, the three domains of eEF2 that form the ligand-binding pocket are oriented in a different manner relative to the rest of eEF2 compared with our previous structure of eEF2 in complex with the parent natural product sordarin. Yeast eEF2 is also shown to bind adenylic nucleotides, which can be displaced by sordarin, suggesting that ADP or ATP also bind to the three C-terminal domains of eEF2. Fusidic acid is a universal inhibitor of translation that targets EF-G or eEF2 and is widely used as an antibiotic against Gram-positive bacteria. Based on mutations conferring resistance to fusidic acid, cryo-EM reconstructions, and x-ray structures of eEF2, EF-G, and an EF-G homolog, we suggest that the conformation of EF-G stalled on the 70 S ribosome by fusidic acid is similar to that of eEF2 trapped on the 80 S ribosome by sordarin.

About this Structure

2NPF is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Sordarin derivatives induce a novel conformation of the yeast ribosome translocation factor eEF2., Soe R, Mosley RT, Justice M, Nielsen-Kahn J, Shastry M, Merrill AR, Andersen GR, J Biol Chem. 2007 Jan 5;282(1):657-66. Epub 2006 Nov 2. PMID:17082187 Page seeded by OCA on Sun May 4 09:44:34 2008

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