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2npx

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[[Image:2npx.jpg|left|200px]]
[[Image:2npx.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2npx |SIZE=350|CAPTION= <scene name='initialview01'>2npx</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_2npx", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CYO:OXYGENS+BOUND+TO+CYS+SG'>CYO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NADH_peroxidase NADH peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.1 1.11.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2npx| PDB=2npx | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npx OCA], [http://www.ebi.ac.uk/pdbsum/2npx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2npx RCSB]</span>
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}}
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'''NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE'''
'''NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE'''
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[[Category: Schulz, G E.]]
[[Category: Schulz, G E.]]
[[Category: Stehle, T.]]
[[Category: Stehle, T.]]
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[[Category: oxidoreductase(h2o2(a))]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:45:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:06:37 2008''
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Revision as of 06:45, 4 May 2008

Image:2npx.jpg

Template:STRUCTURE 2npx

NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE


Overview

The structure of the complex between cofactor NADH and the enzyme NADH peroxidase from Streptococcus faecalis 10C1 (Enterococcus faecalis) has been determined by crystal soaking, X-ray data collection, model building of NADH and refinement at 0.24-nm resolution based on the known enzyme structure [Stehle, T., Ahmed, S. A., Claiborne, A. & Schulz, G. E. (1991) J. Mol. Biol. 221, 1325-1344]. Apart from NADH, the catalytic center of the enzyme contains FAD and a cysteine that shuttles between thiolate and sulfenic acid states. Unfortunately, this cysteine was irreversibly oxidized to a cysteine sulfonic acid in the established enzyme structure. Based on the geometry of the catalytic center, we discuss the stabilization of the oxidation-sensitive sulfenic acid and propose a reaction mechanism.

About this Structure

2NPX is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.

Reference

NADH binding site and catalysis of NADH peroxidase., Stehle T, Claiborne A, Schulz GE, Eur J Biochem. 1993 Jan 15;211(1-2):221-6. PMID:8425532 Page seeded by OCA on Sun May 4 09:45:42 2008

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