2nsf
From Proteopedia
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[[Image:2nsf.gif|left|200px]] | [[Image:2nsf.gif|left|200px]] | ||
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'''Crystal structure of the mycothiol-dependent maleylpyruvate isomerase''' | '''Crystal structure of the mycothiol-dependent maleylpyruvate isomerase''' | ||
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[[Category: Chang, W R.]] | [[Category: Chang, W R.]] | ||
[[Category: Wang, R.]] | [[Category: Wang, R.]] | ||
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Revision as of 06:51, 4 May 2008
Crystal structure of the mycothiol-dependent maleylpyruvate isomerase
Overview
Mycothiol (MSH) is the major low molecular mass thiols in many Gram-positive bacteria such as Mycobacterium tuberculosis and Corynebacterium glutamicum. The physiological roles of MSH are believed to be equivalent to those of GSH in Gram-negative bacteria, but current knowledge of MSH is limited to detoxification of alkalating chemicals and protection from host cell defense/killing systems. Recently, an MSH-dependent maleylpyruvate isomerase (MDMPI) was discovered from C. glutamicum, and this isomerase represents one example of many putative MSH-dependent enzymes that take MSH as cofactor. In this report, fourteen mutants of MDMPI were generated. The wild type and mutant (H52A) MDMPIs were crystallized and their structures were solved at 1.75 and 2.05 A resolution, respectively. The crystal structures reveal that this enzyme contains a divalent metal-binding domain and a C-terminal domain possessing a novel folding pattern (alphabetaalphabetabetaalpha fold). The divalent metal-binding site is composed of residues His52, Glu144, and His148 and is located at the bottom of a surface pocket. Combining the structural and site-directed mutagenesis studies, it is proposed that this surface pocket including the metal ion and MSH moiety formed the putative catalytic center.
About this Structure
2NSF is a Single protein structure of sequence from Corynebacterium glutamicum. Full crystallographic information is available from OCA.
Reference
Crystal structures and site-directed mutagenesis of a mycothiol-dependent enzyme reveal a novel folding and molecular basis for mycothiol-mediated maleylpyruvate isomerization., Wang R, Yin YJ, Wang F, Li M, Feng J, Zhang HM, Zhang JP, Liu SJ, Chang WR, J Biol Chem. 2007 Jun 1;282(22):16288-294. Epub 2007 Apr 11. PMID:17428791 Page seeded by OCA on Sun May 4 09:51:21 2008
