2nuo

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[[Image:2nuo.jpg|left|200px]]
[[Image:2nuo.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2nuo |SIZE=350|CAPTION= <scene name='initialview01'>2nuo</scene>, resolution 1.50&Aring;
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The line below this paragraph, containing "STRUCTURE_2nuo", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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{{STRUCTURE_2nuo| PDB=2nuo | SCENE= }}
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|RELATEDENTRY=[[2gty|2gty]], [[2gux|2gux]], [[2gue|2gue]], [[2guc|2guc]], [[2gud|2gud]], [[2hyr|2hyr]], [[2hyq|2hyq]], [[2i43|2i43]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nuo OCA], [http://www.ebi.ac.uk/pdbsum/2nuo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nuo RCSB]</span>
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'''Crystal structure of a complex of griffithsin with glucose'''
'''Crystal structure of a complex of griffithsin with glucose'''
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[[Category: Wlodawer, A.]]
[[Category: Wlodawer, A.]]
[[Category: Ziolkowska, N E.]]
[[Category: Ziolkowska, N E.]]
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[[Category: domain swapping]]
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[[Category: Domain swapping]]
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[[Category: griffithsin]]
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[[Category: Griffithsin]]
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[[Category: hiv]]
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[[Category: Hiv]]
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[[Category: lectin]]
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[[Category: Lectin]]
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[[Category: mannose binding]]
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[[Category: Mannose binding]]
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[[Category: sar]]
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[[Category: Sar]]
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[[Category: sugar binding protein]]
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[[Category: Sugar binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:56:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:08:46 2008''
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Revision as of 06:56, 4 May 2008

Image:2nuo.jpg

Template:STRUCTURE 2nuo

Crystal structure of a complex of griffithsin with glucose


Overview

Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes.

About this Structure

2NUO is a Single protein structure of sequence from Griffithsia. Full crystallographic information is available from OCA.

Reference

Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736 Page seeded by OCA on Sun May 4 09:56:01 2008

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