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2nuu

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[[Image:2nuu.gif|left|200px]]
[[Image:2nuu.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2nuu |SIZE=350|CAPTION= <scene name='initialview01'>2nuu</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_2nuu", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|GENE= amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), glnK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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{{STRUCTURE_2nuu| PDB=2nuu | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nuu OCA], [http://www.ebi.ac.uk/pdbsum/2nuu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nuu RCSB]</span>
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'''Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex'''
'''Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex'''
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[[Category: Merrick, M.]]
[[Category: Merrick, M.]]
[[Category: Winkler, F K.]]
[[Category: Winkler, F K.]]
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[[Category: ammonia transport]]
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[[Category: Ammonia transport]]
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[[Category: membrane protein complex]]
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[[Category: Membrane protein complex]]
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[[Category: nitrogen regulation]]
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[[Category: Nitrogen regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:56:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:08:43 2008''
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Revision as of 06:56, 4 May 2008

Image:2nuu.gif

Template:STRUCTURE 2nuu

Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex


Overview

Amt proteins are ubiquitous channels for the conduction of ammonia in archaea, eubacteria, fungi, and plants. In Escherichia coli, previous studies have indicated that binding of the PII signal transduction protein GlnK to the ammonia channel AmtB regulates the channel thereby controlling ammonium influx in response to the intracellular nitrogen status. Here, we describe the crystal structure of the complex between AmtB and GlnK at a resolution of 2.5 A. This structure of PII in a complex with one of its targets reveals physiologically relevant conformations of both AmtB and GlnK. GlnK interacts with AmtB almost exclusively via a long surface loop containing Y51 (T-loop), the tip of which inserts deeply into the cytoplasmic pore exit, blocking ammonia conduction. Y51 of GlnK is also buried in the pore exit, explaining why uridylylation of this residue prevents complex formation.

About this Structure

2NUU is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel., Conroy MJ, Durand A, Lupo D, Li XD, Bullough PA, Winkler FK, Merrick M, Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1213-8. Epub 2007 Jan 12. PMID:17220269 Page seeded by OCA on Sun May 4 09:56:11 2008

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