2nwb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2nwb.gif|left|200px]]
[[Image:2nwb.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2nwb |SIZE=350|CAPTION= <scene name='initialview01'>2nwb</scene>, resolution 2.40&Aring;
+
The line below this paragraph, containing "STRUCTURE_2nwb", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= SO_4414 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70863 Shewanella oneidensis])
+
-->
-
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08933 DUF1864]</span>
+
{{STRUCTURE_2nwb| PDB=2nwb | SCENE= }}
-
|RELATEDENTRY=[[1zee|1ZEE]], [[1yw0|1YW0]], [[2nw7|2NW7]], [[2nw8|2NW8]], [[2nw9|2NW9]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nwb OCA], [http://www.ebi.ac.uk/pdbsum/2nwb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nwb RCSB]</span>
+
-
}}
+
'''Crystal Structure of a Putative 2,3-dioxygenase (SO4414) from Shewanella oneidensis in complex with ferric heme. Northeast Structural Genomics Target SoR52.'''
'''Crystal Structure of a Putative 2,3-dioxygenase (SO4414) from Shewanella oneidensis in complex with ferric heme. Northeast Structural Genomics Target SoR52.'''
Line 46: Line 43:
[[Category: Xiao, R.]]
[[Category: Xiao, R.]]
[[Category: Zhao, L.]]
[[Category: Zhao, L.]]
-
[[Category: all alpha-helical protein]]
+
[[Category: All alpha-helical protein]]
-
[[Category: dioxygenase]]
+
[[Category: Dioxygenase]]
-
[[Category: heme-binding protein]]
+
[[Category: Heme-binding protein]]
-
[[Category: hemoprotein]]
+
[[Category: Hemoprotein]]
-
[[Category: nesg]]
+
[[Category: Nesg]]
-
[[Category: northeast structural genomics consortium]]
+
[[Category: Northeast structural genomics consortium]]
-
[[Category: protein structure initiative]]
+
[[Category: Protein structure initiative]]
-
[[Category: psi-2]]
+
[[Category: Psi-2]]
-
[[Category: structural genomic]]
+
[[Category: Structural genomic]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:59:27 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:09:16 2008''
+

Revision as of 06:59, 4 May 2008

Image:2nwb.gif

Template:STRUCTURE 2nwb

Crystal Structure of a Putative 2,3-dioxygenase (SO4414) from Shewanella oneidensis in complex with ferric heme. Northeast Structural Genomics Target SoR52.


Overview

Tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) constitute an important, yet relatively poorly understood, family of heme-containing enzymes. Here, we report extensive structural and biochemical studies of the Xanthomonas campestris TDO and a related protein SO4414 from Shewanella oneidensis, including the structure at 1.6-A resolution of the catalytically active, ferrous form of TDO in a binary complex with the substrate L-Trp. The carboxylate and ammonium moieties of tryptophan are recognized by electrostatic and hydrogen-bonding interactions with the enzyme and a propionate group of the heme, thus defining the L-stereospecificity. A second, possibly allosteric, L-Trp-binding site is present at the tetramer interface. The sixth coordination site of the heme-iron is vacant, providing a dioxygen-binding site that would also involve interactions with the ammonium moiety of L-Trp and the amide nitrogen of a glycine residue. The indole ring is positioned correctly for oxygenation at the C2 and C3 atoms. The active site is fully formed only in the binary complex, and biochemical experiments confirm this induced-fit behavior of the enzyme. The active site is completely devoid of water during catalysis, which is supported by our electrochemical studies showing significant stabilization of the enzyme upon substrate binding.

About this Structure

2NWB is a Single protein structure of sequence from Shewanella oneidensis. Full crystallographic information is available from OCA.

Reference

Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase., Forouhar F, Anderson JL, Mowat CG, Vorobiev SM, Hussain A, Abashidze M, Bruckmann C, Thackray SJ, Seetharaman J, Tucker T, Xiao R, Ma LC, Zhao L, Acton TB, Montelione GT, Chapman SK, Tong L, Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):473-8. Epub 2006 Dec 29. PMID:17197414 Page seeded by OCA on Sun May 4 09:59:27 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2nwb"
Personal tools