2nzg

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[[Image:2nzg.gif|left|200px]]
[[Image:2nzg.gif|left|200px]]
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{{Structure
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|PDB= 2nzg |SIZE=350|CAPTION= <scene name='initialview01'>2nzg</scene>, resolution 1.94&Aring;
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The line below this paragraph, containing "STRUCTURE_2nzg", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>
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{{STRUCTURE_2nzg| PDB=2nzg | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzg OCA], [http://www.ebi.ac.uk/pdbsum/2nzg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nzg RCSB]</span>
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'''Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details'''
'''Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details'''
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==About this Structure==
==About this Structure==
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2NZG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. This structure supersedes the now removed PDB entry 1TL0. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZG OCA].
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This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZG OCA].
==Reference==
==Reference==
Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin: 1.9 A crystal structure reveals the details., Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U, Structure. 2004 Sep;12(9):1655-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341730 15341730]
Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin: 1.9 A crystal structure reveals the details., Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U, Structure. 2004 Sep;12(9):1655-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341730 15341730]
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[[Category: Protein complex]]
 
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[[Category: Vibrio cholerae]]
 
[[Category: Angstrom, J.]]
[[Category: Angstrom, J.]]
[[Category: Holmner, A.]]
[[Category: Holmner, A.]]
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[[Category: Okvist, M.]]
[[Category: Okvist, M.]]
[[Category: Teneberg, S.]]
[[Category: Teneberg, S.]]
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[[Category: blood group antigen]]
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[[Category: Blood group antigen]]
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[[Category: cholera toxin]]
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[[Category: Cholera toxin]]
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[[Category: heat-labile enterotoxin]]
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[[Category: Heat-labile enterotoxin]]
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[[Category: protein-carbohydrate complex]]
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[[Category: Protein-carbohydrate complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:06:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:10:36 2008''
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Revision as of 07:06, 4 May 2008

Image:2nzg.gif

Template:STRUCTURE 2nzg

Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details


Overview

A hybrid between the B subunits of cholera toxin and Escherichia coli heat-labile enterotoxin has been described, which exhibits a novel binding specificity to blood group A and B type 2 determinants. In the present investigation, we have determined the crystal structure of this protein hybrid, termed LCTBK, in complex with the blood group A pentasaccharide GalNAcalpha3(Fucalpha2)Galbeta4(Fucalpha3)GlcNAcbeta, confirming not only the novel binding specificity but also a distinct new oligosaccharide binding site. Binding studies revealed that the new specificity can be ascribed to a single mutation (S4N) introduced into the sequence of Escherichia coli heat-labile enterotoxin. At a resolution of 1.9 A, the new binding site is resolved in excellent detail. Main features include a complex network of water molecules, which is well preserved by the parent toxins, and an unexpectedly modest contribution to binding by the critical residue Asn4, which interacts with the ligand only via a single water molecule.

About this Structure

This structure supersedes the now removed PDB entry 1tl0. Full crystallographic information is available from OCA.

Reference

Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin: 1.9 A crystal structure reveals the details., Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U, Structure. 2004 Sep;12(9):1655-67. PMID:15341730 Page seeded by OCA on Sun May 4 10:06:59 2008

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