2nzo
From Proteopedia
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[[Image:2nzo.gif|left|200px]] | [[Image:2nzo.gif|left|200px]] | ||
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'''Crystal structure of a secretion chaperone CsaA from Bacillus subtilis in the space group P 32 2 1''' | '''Crystal structure of a secretion chaperone CsaA from Bacillus subtilis in the space group P 32 2 1''' | ||
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[[Category: Paetzel, M.]] | [[Category: Paetzel, M.]] | ||
[[Category: Shapova, Y A.]] | [[Category: Shapova, Y A.]] | ||
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| - | [[Category: | + | [[Category: Ob fold]] |
| - | [[Category: | + | [[Category: Oligonucleotide/oligosaccharide binding fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:07:18 2008'' | |
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Revision as of 07:07, 4 May 2008
Crystal structure of a secretion chaperone CsaA from Bacillus subtilis in the space group P 32 2 1
Overview
Bacillus subtilis CsaA (BsCsaA) has been proposed to act as a protein-secretion chaperone in the Sec-dependent translocation pathway, possibly compensating for the lack of SecB in the Gram-positive eubacterium Bacillus subtilis. This paper presents the cloning, purification, crystallization and structures of BsCsaA in two space groups (P42(1)2 and P3(2)21) solved and refined to resolutions of 1.9 and 2.0 A, respectively. These structures complement the previously available crystal structure of CsaA from the Gram-negative eubacterium Thermus thermophilus (TtCsaA) and provide a direct structural basis for the interpretation of previously available biochemical data on BsCsaA. The sequence and structure of the proposed substrate-binding pocket are analyzed and discussed. A comparison with the TtCsaA structure reveals a different pattern of electrostatic potential in the vicinity of the binding site, which overlaps with a region of high sequence variability. In addition, the dimerization interface of this homodimeric protein is analyzed and discussed.
About this Structure
2NZO is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Crystallographic analysis of Bacillus subtilis CsaA., Shapova YA, Paetzel M, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):478-85. Epub 2007, Mar 16. PMID:17372352 Page seeded by OCA on Sun May 4 10:07:18 2008
