2o1c
From Proteopedia
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'''Structure of the E. coli dihydroneopterin triphosphate pyrophosphohydrolase''' | '''Structure of the E. coli dihydroneopterin triphosphate pyrophosphohydrolase''' | ||
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[[Category: Bianchet, M A.]] | [[Category: Bianchet, M A.]] | ||
[[Category: Gabelli, S B.]] | [[Category: Gabelli, S B.]] | ||
| - | [[Category: | + | [[Category: Nudix ntp hydrolase ntp pyrophosphohydrolase mutt dihydroneopterin triphosphate pyrophosphohydrolase folate biosynthesis]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:11:14 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:11, 4 May 2008
Structure of the E. coli dihydroneopterin triphosphate pyrophosphohydrolase
Overview
The product of the Escherichia coli orf17 gene is a novel nucleoside triphosphate pyrophosphohydrolase with a preference for dATP over the other canonical (deoxy)nucleoside triphosphates, and it catalyzes the hydrolysis of dATP through a nucleophilic attack at the beta-phosphorus to produce dAMP and inorganic pyrophosphate. It has a pH optimum between 8.5 and 9.0, a divalent metal ion requirement with optimal activity at 5 mM Mg2+, a Km of 0.8 mM and a kcat of 5.2 s-1 at 37 degrees C for dATP. dAMP is a weak competitive inhibitor with a Ki of approximately 4 mM, while PPi is a much stronger inhibitor with an apparent Ki of approximately 20 microM. The enzyme contains the highly conserved signature sequence GXVEX2ETX6REVXEEX2I designating the MutT family of proteins. However, unlike the other nucleoside triphosphate pyrophosphohydrolases with this conserved sequence, the Orf17 protein does not complement the mutT- mutator phenotype, and thus must serve a different biological role in the cell.
About this Structure
2O1C is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Escherichia coli orf17 codes for a nucleoside triphosphate pyrophosphohydrolase member of the MutT family of proteins. Cloning, purification, and characterization of the enzyme., O'Handley SF, Frick DN, Bullions LC, Mildvan AS, Bessman MJ, J Biol Chem. 1996 Oct 4;271(40):24649-54. PMID:8798731 Page seeded by OCA on Sun May 4 10:11:14 2008
