2o1v

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2o1v.jpg|left|200px]]
[[Image:2o1v.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2o1v |SIZE=350|CAPTION= <scene name='initialview01'>2o1v</scene>, resolution 2.45&Aring;
+
The line below this paragraph, containing "STRUCTURE_2o1v", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= HSP90B1, TRA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 Canis lupus familiaris])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2o1v| PDB=2o1v | SCENE= }}
-
|RELATEDENTRY=[[2o1u|2O1U]], [[2o1w|2O1W]], [[2o1t|2O1T]], [[1tc6|1TC6]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o1v OCA], [http://www.ebi.ac.uk/pdbsum/2o1v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o1v RCSB]</span>
+
-
}}
+
'''Structure of full length GRP94 with ADP bound'''
'''Structure of full length GRP94 with ADP bound'''
Line 29: Line 26:
[[Category: Immormino, R M.]]
[[Category: Immormino, R M.]]
[[Category: Warren, J J.]]
[[Category: Warren, J J.]]
-
[[Category: adp]]
+
[[Category: Adp]]
-
[[Category: chaperone]]
+
[[Category: Chaperone]]
-
[[Category: endoplasmin,]]
+
[[Category: Endoplasmin]]
-
[[Category: gp96]]
+
[[Category: Gp96]]
-
[[Category: grp94]]
+
[[Category: Grp94]]
-
[[Category: hsp82]]
+
[[Category: Hsp82]]
-
[[Category: hsp90]]
+
[[Category: Hsp90]]
-
[[Category: htpg]]
+
[[Category: Htpg]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:12:33 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:11:31 2008''
+

Revision as of 07:12, 4 May 2008

Image:2o1v.jpg

Template:STRUCTURE 2o1v

Structure of full length GRP94 with ADP bound


Overview

GRP94, an essential endoplasmic reticulum chaperone, is required for the conformational maturation of proteins destined for cell-surface display or export. The extent to which GRP94 and its cytosolic paralog, Hsp90, share a common mechanism remains controversial. GRP94 has not been shown conclusively to hydrolyze ATP or bind cochaperones, and both activities, by contrast, result in conformational changes and N-terminal dimerization in Hsp90 that are critical for its function. Here, we report the 2.4 A crystal structure of mammalian GRP94 in complex with AMPPNP and ADP. The chaperone is conformationally insensitive to the identity of the bound nucleotide, adopting a "twisted V" conformation that precludes N-terminal domain dimerization. We also present conclusive evidence that GRP94 possesses ATPase activity. Our observations provide a structural explanation for GRP94's observed rate of ATP hydrolysis and suggest a model for the role of ATP binding and hydrolysis in the GRP94 chaperone cycle.

About this Structure

2O1V is a Single protein structure of sequence from Canis lupus familiaris. Full crystallographic information is available from OCA.

Reference

Structures of GRP94-nucleotide complexes reveal mechanistic differences between the hsp90 chaperones., Dollins DE, Warren JJ, Immormino RM, Gewirth DT, Mol Cell. 2007 Oct 12;28(1):41-56. PMID:17936703 Page seeded by OCA on Sun May 4 10:12:33 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2o1v"
Personal tools