2o2l
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2o2l.jpg|left|200px]] | [[Image:2o2l.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2o2l", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_2o2l| PDB=2o2l | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of human heat-labile enterotoxin in complex with a blood group A antigen analog''' | '''Crystal structure of human heat-labile enterotoxin in complex with a blood group A antigen analog''' | ||
| Line 29: | Line 26: | ||
[[Category: Krengel, U.]] | [[Category: Krengel, U.]] | ||
[[Category: Okvist, M.]] | [[Category: Okvist, M.]] | ||
| - | [[Category: | + | [[Category: Blood group antigen]] |
| - | [[Category: | + | [[Category: Enterotoxigenic e. coli]] |
| - | [[Category: | + | [[Category: Heat-labile enterotoxin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:14:35 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:14, 4 May 2008
Crystal structure of human heat-labile enterotoxin in complex with a blood group A antigen analog
Overview
In a number of bacterial infections, such as Helicobacter pylori, Campylobacter jejuni and Vibrio cholerae infections, a correlation between the severity of disease and blood group phenotype of infected individuals has been observed. In the present investigation, we have studied the molecular basis of this effect for enterotoxigenic Escherichia coli (ETEC) infections. ETEC are non-invasive bacteria, which act through second messenger pathways to cause diarrhea. It has been suggested that the major virulence factor of ETEC from human isolates, i.e. the human heat-labile enterotoxin (hLT), recognizes certain blood group epitopes, although the molecular basis of blood group antigen recognition is unknown. The 2.5 A crystal structure of the receptor-binding B-subunit of hLT in complex with the blood group A antigen analog GalNAcalpha3(Fucalpha2)Galbeta4(Fucalpha3)Glcbeta provides evidence of a previously unknown binding site in the native toxin. The structure reveals the molecular interactions underlying blood group antigen recognition and suggests how this protein can discriminate between different blood group epitopes. These results support the previously debated role of hLT in the blood group dependence of ETEC infections. Similar observations regarding the closely related cholera toxin in V. cholera infections are also discussed.
About this Structure
2O2L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Blood group antigen recognition by Escherichia coli heat-labile enterotoxin., Holmner A, Askarieh G, Okvist M, Krengel U, J Mol Biol. 2007 Aug 17;371(3):754-64. Epub 2007 May 31. PMID:17586525 Page seeded by OCA on Sun May 4 10:14:35 2008
