2o5i

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[[Image:2o5i.gif|left|200px]]
[[Image:2o5i.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2o5i |SIZE=350|CAPTION= <scene name='initialview01'>2o5i</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_2o5i", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2o5i| PDB=2o5i | SCENE= }}
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|RELATEDENTRY=[[2o5j|2O5J]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5i OCA], [http://www.ebi.ac.uk/pdbsum/2o5i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o5i RCSB]</span>
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}}
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'''Crystal structure of the T. thermophilus RNA polymerase elongation complex'''
'''Crystal structure of the T. thermophilus RNA polymerase elongation complex'''
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[[Category: Vassylyev, D G.]]
[[Category: Vassylyev, D G.]]
[[Category: Vassylyeva, M N.]]
[[Category: Vassylyeva, M N.]]
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[[Category: elongation complex]]
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[[Category: Elongation complex]]
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[[Category: non-template dna]]
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[[Category: Non-template dna]]
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[[Category: rna polymerase]]
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[[Category: Rna polymerase]]
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[[Category: rna transcript]]
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[[Category: Rna transcript]]
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[[Category: template dna]]
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[[Category: Template dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:21:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:12:58 2008''
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Revision as of 07:21, 4 May 2008

Image:2o5i.gif

Template:STRUCTURE 2o5i

Crystal structure of the T. thermophilus RNA polymerase elongation complex


Overview

The RNA polymerase elongation complex (EC) is both highly stable and processive, rapidly extending RNA chains for thousands of nucleotides. Understanding the mechanisms of elongation and its regulation requires detailed information about the structural organization of the EC. Here we report the 2.5-A resolution structure of the Thermus thermophilus EC; the structure reveals the post-translocated intermediate with the DNA template in the active site available for pairing with the substrate. DNA strand separation occurs one position downstream of the active site, implying that only one substrate at a time can specifically bind to the EC. The upstream edge of the RNA/DNA hybrid stacks on the beta'-subunit 'lid' loop, whereas the first displaced RNA base is trapped within a protein pocket, suggesting a mechanism for RNA displacement. The RNA is threaded through the RNA exit channel, where it adopts a conformation mimicking that of a single strand within a double helix, providing insight into a mechanism for hairpin-dependent pausing and termination.

About this Structure

2O5I is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structural basis for transcription elongation by bacterial RNA polymerase., Vassylyev DG, Vassylyeva MN, Perederina A, Tahirov TH, Artsimovitch I, Nature. 2007 Jul 12;448(7150):157-62. Epub 2007 Jun 20. PMID:17581590 Page seeded by OCA on Sun May 4 10:21:07 2008

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