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2o5v
From Proteopedia
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[[Image:2o5v.gif|left|200px]] | [[Image:2o5v.gif|left|200px]] | ||
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'''Recombination mediator RecF''' | '''Recombination mediator RecF''' | ||
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[[Category: Korolev, S.]] | [[Category: Korolev, S.]] | ||
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Revision as of 07:22, 4 May 2008
Recombination mediator RecF
Overview
RecF, together with RecO and RecR, belongs to a ubiquitous group of recombination mediators (RMs) that includes eukaryotic proteins such as Rad52 and BRCA2. RMs help maintain genome stability in the presence of DNA damage by loading RecA-like recombinases and displacing single-stranded DNA-binding proteins. Here, we present the crystal structure of RecF from Deinococcus radiodurans. RecF exhibits a high degree of structural similarity with the head domain of Rad50, but lacks its long coiled-coil region. The structural homology between RecF and Rad50 is extensive, encompassing the ATPase subdomain and the so-called 'Lobe II' subdomain of Rad50. The pronounced structural conservation between bacterial RecF and evolutionarily diverged eukaryotic Rad50 implies a conserved mechanism of DNA binding and recognition of the boundaries of double-stranded DNA regions. The RecF structure, mutagenesis of conserved motifs and ATP-dependent dimerization of RecF are discussed with respect to its role in promoting presynaptic complex formation at DNA damage sites.
About this Structure
2O5V is a Single protein structure of sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA.
Reference
Structural conservation of RecF and Rad50: implications for DNA recognition and RecF function., Koroleva O, Makharashvili N, Courcelle CT, Courcelle J, Korolev S, EMBO J. 2007 Feb 7;26(3):867-77. Epub 2007 Jan 25. PMID:17255941 Page seeded by OCA on Sun May 4 10:22:00 2008
