2o7f

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[[Image:2o7f.jpg|left|200px]]
[[Image:2o7f.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2o7f |SIZE=350|CAPTION= <scene name='initialview01'>2o7f</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_2o7f", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=HC4:4&#39;-HYDROXYCINNAMIC+ACID'>HC4</scene>, <scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-5-HYDROXY-4-METHYL-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= hutH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides])
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-->
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|DOMAIN=
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{{STRUCTURE_2o7f| PDB=2o7f | SCENE= }}
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|RELATEDENTRY=[[2o6y|2o6y]], [[1gkm|1gkm]], [[1w27|1w27]], [[1y2m|1y2m]], [[1t6j|1t6j]], [[2nyn|2nyn]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7f OCA], [http://www.ebi.ac.uk/pdbsum/2o7f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o7f RCSB]</span>
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}}
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'''Tyrosine ammonia-lyase from Rhodobacter sphaeroides (His89Phe variant), complexed with coumaric acid'''
'''Tyrosine ammonia-lyase from Rhodobacter sphaeroides (His89Phe variant), complexed with coumaric acid'''
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[[Category: Moore, B S.]]
[[Category: Moore, B S.]]
[[Category: Noel, J P.]]
[[Category: Noel, J P.]]
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[[Category: methylidene imidazolone prosthetic group]]
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[[Category: Methylidene imidazolone prosthetic group]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:24:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:13:57 2008''
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Revision as of 07:24, 4 May 2008

Image:2o7f.jpg

Template:STRUCTURE 2o7f

Tyrosine ammonia-lyase from Rhodobacter sphaeroides (His89Phe variant), complexed with coumaric acid


Overview

Aromatic amino acid ammonia-lyases catalyze the deamination of L-His, L-Phe, and L-Tyr, yielding ammonia plus aryl acids bearing an alpha,beta-unsaturated propenoic acid. We report crystallographic analyses of unliganded Rhodobacter sphaeroides tyrosine ammonia-lyase (RsTAL) and RsTAL bound to p-coumarate and caffeate. His 89 of RsTAL forms a hydrogen bond with the p-hydroxyl moieties of coumarate and caffeate. His 89 is conserved in TALs but replaced in phenylalanine ammonia-lyases (PALs) and histidine ammonia-lyases (HALs). Substitution of His 89 by Phe, a characteristic residue of PALs, yields a mutant with a switch in kinetic preference from L-Tyr to L-Phe. Structures of the H89F mutant in complex with the PAL product, cinnamate, or the PAL-specific inhibitor, 2-aminoindan-2-phosphonate (AIP), support the role of position 89 as a specificity determinant in the family of aromatic amino acid ammonia-lyases and aminomutases responsible for beta-amino acid biosynthesis.

About this Structure

2O7F is a Single protein structure of sequence from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

Reference

Structural determinants and modulation of substrate specificity in phenylalanine-tyrosine ammonia-lyases., Louie GV, Bowman ME, Moffitt MC, Baiga TJ, Moore BS, Noel JP, Chem Biol. 2006 Dec;13(12):1327-38. PMID:17185228 Page seeded by OCA on Sun May 4 10:24:54 2008

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