2o8k

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[[Image:2o8k.jpg|left|200px]]
[[Image:2o8k.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2o8k |SIZE=350|CAPTION= <scene name='initialview01'>2o8k</scene>
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The line below this paragraph, containing "STRUCTURE_2o8k", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= rpoN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])
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-->
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|DOMAIN=
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{{STRUCTURE_2o8k| PDB=2o8k | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o8k OCA], [http://www.ebi.ac.uk/pdbsum/2o8k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o8k RCSB]</span>
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}}
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'''NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element'''
'''NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element'''
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[[Category: Pelton, J G.]]
[[Category: Pelton, J G.]]
[[Category: Wemmer, D E.]]
[[Category: Wemmer, D E.]]
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[[Category: helix-turn-helix]]
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[[Category: Helix-turn-helix]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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[[Category: rna polymerase]]
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[[Category: Rna polymerase]]
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[[Category: sigma-54]]
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[[Category: Sigma-54]]
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[[Category: transcription factor]]
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[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:27:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:14:17 2008''
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Revision as of 07:27, 4 May 2008

Image:2o8k.jpg

Template:STRUCTURE 2o8k

NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element


Overview

The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP.

About this Structure

2O8K is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

Reference

Structural basis of DNA recognition by the alternative sigma-factor, sigma54., Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE, J Mol Biol. 2007 Jun 15;369(4):1070-8. Epub 2007 Apr 12. PMID:17481658 Page seeded by OCA on Sun May 4 10:27:18 2008

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