This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2o9l
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2o9l.jpg|left|200px]] | [[Image:2o9l.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2o9l", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2o9l| PDB=2o9l | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element''' | '''AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element''' | ||
| Line 29: | Line 26: | ||
[[Category: Pelton, J G.]] | [[Category: Pelton, J G.]] | ||
[[Category: Wemmer, D E.]] | [[Category: Wemmer, D E.]] | ||
| - | [[Category: | + | [[Category: Amber]] |
| - | [[Category: | + | [[Category: Generalized born solvent model]] |
| - | [[Category: | + | [[Category: Helix-turn-helix]] |
| - | [[Category: | + | [[Category: Protein-dna complex]] |
| - | [[Category: | + | [[Category: Rna polymerase]] |
| - | [[Category: | + | [[Category: Sigma-54]] |
| - | [[Category: | + | [[Category: Transcription factor]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:29:41 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:29, 4 May 2008
AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element
Overview
The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP.
About this Structure
2O9L is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Structural basis of DNA recognition by the alternative sigma-factor, sigma54., Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE, J Mol Biol. 2007 Jun 15;369(4):1070-8. Epub 2007 Apr 12. PMID:17481658 Page seeded by OCA on Sun May 4 10:29:41 2008
