1ohg
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(New page: 200px<br /> <applet load="1ohg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ohg, resolution 3.45Å" /> '''STRUCTURE OF THE DS...)
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Revision as of 06:57, 18 November 2007
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STRUCTURE OF THE DSDNA BACTERIOPHAGE HK97 MATURE EMPTY CAPSID
Overview
The HK97 bacteriophage capsid is a unique example of macromolecular, catenanes: interlocked rings of covalently attached protein subunits. The, chain mail organization of the subunits stabilizes a particle in which the, maximum thickness of the protein shell is 18A and the maximum diameter is, 550A. The electron density has the appearance of a balloon illustrating, the extraordinary strength conferred by the unique subunit organization., The refined structure shows novel qualities of the HK97 shell protein, gp5, that, together with the protease gp4, guides the assembly and maturation, of the virion. Although gp5 forms hexamers and pentamers and the subunits, exist in different structural environments, the tertiary structures of the, seven protein molecules in the viral asymmetric unit are closely similar., The interactions of the subunits in the shell are exceptionally complex, with each subunit interacting with nine other subunits. The interactions, of the N-terminus released after gp5 cleavage appear important for, organization of the loops that become crosslinked to the core of a, neighboring subunit at the maturation. A comparison with a model of the, Prohead II structure revealed that the surfaces of non-covalent contact, between the monomers that build up hexamers/pentamers are completely, redefined during maturation.
About this Structure
1OHG is a Single protein structure of sequence from Enterobacteria phage hk620 with SO4 and CL as ligands. This structure superseeds the now removed PDB entry 1FH6. The following page contains interesting information on the relation of 1OHG with [Citrate Synthase]. Full crystallographic information is available from OCA.
Reference
The refined structure of a protein catenane: the HK97 bacteriophage capsid at 3.44 A resolution., Helgstrand C, Wikoff WR, Duda RL, Hendrix RW, Johnson JE, Liljas L, J Mol Biol. 2003 Dec 12;334(5):885-99. PMID:14643655
Page seeded by OCA on Sun Nov 18 09:04:26 2007
