2oga
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2oga.gif|left|200px]] | [[Image:2oga.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2oga", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2oga| PDB=2oga | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''X-ray crystal structure of S. venezuelae DesV in complex with ketimine intermediate''' | '''X-ray crystal structure of S. venezuelae DesV in complex with ketimine intermediate''' | ||
| Line 27: | Line 24: | ||
[[Category: Burgie, E S.]] | [[Category: Burgie, E S.]] | ||
[[Category: Holden, H M.]] | [[Category: Holden, H M.]] | ||
| - | [[Category: | + | [[Category: Antibiotic]] |
| - | [[Category: | + | [[Category: Deoxysugar]] |
| - | [[Category: | + | [[Category: Desosamine]] |
| - | [[Category: | + | [[Category: Plp-dependent enzyme]] |
| - | [[Category: | + | [[Category: Transaminase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:50:58 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:51, 4 May 2008
X-ray crystal structure of S. venezuelae DesV in complex with ketimine intermediate
Overview
Desosamine is a 3-(dimethylamino)-3,4,6-trideoxyhexose found in certain macrolide antibiotics such as the commonly prescribed erythromycin. Six enzymes are required for its biosynthesis in Streptomyces venezuelae. The focus of this article is DesV, which catalyzes the PLP-dependent replacement of a 3-keto group with an amino functionality in the fifth step of the pathway. For this study the three-dimensional structures of both the internal aldimine and the ketimine intermediate with glutamate were determined to 2.05 A resolution. DesV is a homodimer with each subunit containing 12 alpha-helical regions and 12 beta-strands that together form three layers of sheet. The structure of the internal aldimine demonstrates that the PLP-cofactor is held in place by residues contributed from both subunits (Asp 164 and Gln 167 from Subunit I and Tyr 221 and Asn 235 from Subunit II). When the ketimine intermediate is present in the active site, the loop defined by Gln 225 to Ser 228 from Subunit II closes down upon the active site. The structure of DesV is similar to another sugar-modifying enzyme referred to as PseC. This enzyme is involved in the biosynthesis of pseudaminic acid, which is a sialic acid-like nonulosonate found in the flagellin of Helicobacter pylori. In the case of PseC, however, the amino group is transferred to the C-4 rather than the C-3 position. Details concerning the structural analysis of DesV and a comparison of its molecular architecture to that of PseC are presented.
About this Structure
2OGA is a Single protein structure of sequence from Streptomyces venezuelae. Full crystallographic information is available from OCA.
Reference
Molecular architecture of DesV from Streptomyces venezuelae: a PLP-dependent transaminase involved in the biosynthesis of the unusual sugar desosamine., Burgie ES, Thoden JB, Holden HM, Protein Sci. 2007 May;16(5):887-96. PMID:17456741 Page seeded by OCA on Sun May 4 10:50:58 2008
