2oi2

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[[Image:2oi2.jpg|left|200px]]
[[Image:2oi2.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2oi2 |SIZE=350|CAPTION= <scene name='initialview01'>2oi2</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_2oi2", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DP6:(3R)-3-HYDROXY-5-{[(R)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}-3-METHYLPENTANOIC+ACID'>DP6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Mevalonate_kinase Mevalonate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.36 2.7.1.36] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= mvk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])
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|DOMAIN=
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{{STRUCTURE_2oi2| PDB=2oi2 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oi2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oi2 OCA], [http://www.ebi.ac.uk/pdbsum/2oi2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oi2 RCSB]</span>
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'''Streptococcus pneumoniae Mevalonate Kinase in Complex with Diphosphomevalonate'''
'''Streptococcus pneumoniae Mevalonate Kinase in Complex with Diphosphomevalonate'''
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[[Category: Roderick, S L.]]
[[Category: Roderick, S L.]]
[[Category: Vetting, M W.]]
[[Category: Vetting, M W.]]
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[[Category: enzyme-inhibitor complex]]
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[[Category: Enzyme-inhibitor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:57:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:18:09 2008''
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Revision as of 07:58, 4 May 2008

Image:2oi2.jpg

Template:STRUCTURE 2oi2

Streptococcus pneumoniae Mevalonate Kinase in Complex with Diphosphomevalonate


Overview

Streptococcus pneumoniae, a ubiquitous gram-positive pathogen with an alarming, steadily evolving resistance to frontline antimicrobials, poses a severe global health threat both in the community and in the clinic. The recent discovery that diphosphomevalonate (DPM), an essential intermediate in the isoprenoid biosynthetic pathway, potently and allosterically inhibits S. pneumoniae mevalonate kinase (SpMK) without affecting the human isozyme established a new target and lead compound for antimicrobial design. Here we present the crystal structure of the first S. pneumoniae mevalonate kinase, at a resolution of 2.5 A and in complex with DPM.Mg(2+) in the active-site cleft. Structural comparison of SpMK with other members of the GHMP kinase family reveals that DPM functions as a partial bisubstrate analog (mevalonate linked to the pyrophosphoryl moiety of ATP) in that it elicits a ternary-complexlike form of the enzyme, except for localized disordering in a region that would otherwise interact with the missing portion of the nucleotide. Features of the SpMK-binding pockets are discussed in the context of established mechanistic findings and inherited human diseases linked to MK deficiency.

About this Structure

2OI2 is a Single protein structure of sequence from Streptococcus pneumoniae. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Streptococcus pneumoniae mevalonate kinase in complex with diphosphomevalonate., Andreassi JL 2nd, Bilder PW, Vetting MW, Roderick SL, Leyh TS, Protein Sci. 2007 May;16(5):983-9. Epub 2007 Mar 30. PMID:17400916 Page seeded by OCA on Sun May 4 10:57:57 2008

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