2oiu
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2oiu.gif|left|200px]] | [[Image:2oiu.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2oiu", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_2oiu| PDB=2oiu | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''L1 Ribozyme Ligase circular adduct''' | '''L1 Ribozyme Ligase circular adduct''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OIU OCA]. | |
==Reference== | ==Reference== | ||
The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17363667 17363667] | The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17363667 17363667] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Robertson, M P]] | [[Category: Robertson, M P]] | ||
[[Category: Scott, W G.]] | [[Category: Scott, W G.]] | ||
| - | [[Category: | + | [[Category: Active site]] |
| - | [[Category: | + | [[Category: In vitro selection]] |
| - | [[Category: | + | [[Category: Ligase]] |
| - | [[Category: | + | [[Category: Ribozyme]] |
| - | [[Category: | + | [[Category: Rna]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:00:21 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:00, 4 May 2008
L1 Ribozyme Ligase circular adduct
Overview
Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.
About this Structure
Full crystallographic information is available from OCA.
Reference
The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:17363667 Page seeded by OCA on Sun May 4 11:00:21 2008
