2okh
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2okh.gif|left|200px]] | [[Image:2okh.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2okh", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2okh| PDB=2okh | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of dimeric form of PfFabZ in crystal form3''' | '''Crystal structure of dimeric form of PfFabZ in crystal form3''' | ||
| Line 31: | Line 28: | ||
[[Category: Surolia, N.]] | [[Category: Surolia, N.]] | ||
[[Category: Swarnamukhi, P L.]] | [[Category: Swarnamukhi, P L.]] | ||
| - | [[Category: | + | [[Category: Fabz]] |
| - | [[Category: | + | [[Category: Hotdog fold]] |
| - | [[Category: | + | [[Category: Non-isomorphism]] |
| - | [[Category: | + | [[Category: Plasmodium]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:05:55 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:05, 4 May 2008
Crystal structure of dimeric form of PfFabZ in crystal form3
Overview
The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited non-isomorphism. The c axis of the I222 cell showed a large variation of about 16 A, from c = 81 A to c = 97 A. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, c = 97 A; form 2, c = 92 A; form 3, c = 81 A). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences.
About this Structure
2OKH is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum., Swarnamukhi PL, Sharma SK, Padala P, Surolia N, Surolia A, Suguna K, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):458-64. Epub 2007, Mar 16. PMID:17372349 Page seeded by OCA on Sun May 4 11:05:55 2008
