2okj

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[[Image:2okj.gif|left|200px]]
[[Image:2okj.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2okj |SIZE=350|CAPTION= <scene name='initialview01'>2okj</scene>, resolution 2.300&Aring;
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The line below this paragraph, containing "STRUCTURE_2okj", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ABU:GAMMA-AMINO-BUTANOIC+ACID'>ABU</scene>, <scene name='pdbligand=LLP:2-LYSINE(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHANE)'>LLP</scene>, <scene name='pdbligand=PLZ:4-[({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)AMINO]BUTANOIC+ACID'>PLZ</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate_decarboxylase Glutamate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.15 4.1.1.15] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= GAD1, GAD, GAD67 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2okj| PDB=2okj | SCENE= }}
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|RELATEDENTRY=[[2okk|2OKK]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2okj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2okj OCA], [http://www.ebi.ac.uk/pdbsum/2okj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2okj RCSB]</span>
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}}
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'''The X-ray crystal structure of the 67kDa isoform of Glutamic Acid Decarboxylase (GAD67)'''
'''The X-ray crystal structure of the 67kDa isoform of Glutamic Acid Decarboxylase (GAD67)'''
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[[Category: Law, R H.P.]]
[[Category: Law, R H.P.]]
[[Category: Whisstock, J C.]]
[[Category: Whisstock, J C.]]
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[[Category: plp-dependent decarboxylase]]
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[[Category: Plp-dependent decarboxylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:06:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:19:09 2008''
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Revision as of 08:06, 4 May 2008

Image:2okj.gif

Template:STRUCTURE 2okj

The X-ray crystal structure of the 67kDa isoform of Glutamic Acid Decarboxylase (GAD67)


Overview

Gamma-aminobutyric acid (GABA) is synthesized by two isoforms of the pyridoxal 5'-phosphate-dependent enzyme glutamic acid decarboxylase (GAD65 and GAD67). GAD67 is constitutively active and is responsible for basal GABA production. In contrast, GAD65, an autoantigen in type I diabetes, is transiently activated in response to the demand for extra GABA in neurotransmission, and cycles between an active holo form and an inactive apo form. We have determined the crystal structures of N-terminal truncations of both GAD isoforms. The structure of GAD67 shows a tethered loop covering the active site, providing a catalytic environment that sustains GABA production. In contrast, the same catalytic loop is inherently mobile in GAD65. Kinetic studies suggest that mobility in the catalytic loop promotes a side reaction that results in cofactor release and GAD65 autoinactivation. These data reveal the molecular basis for regulation of GABA homeostasis.

About this Structure

2OKJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop., Fenalti G, Law RH, Buckle AM, Langendorf C, Tuck K, Rosado CJ, Faux NG, Mahmood K, Hampe CS, Banga JP, Wilce M, Schmidberger J, Rossjohn J, El-Kabbani O, Pike RN, Smith AI, Mackay IR, Rowley MJ, Whisstock JC, Nat Struct Mol Biol. 2007 Apr;14(4):280-6. Epub 2007 Mar 25. PMID:17384644 Page seeded by OCA on Sun May 4 11:06:05 2008

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OCA

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