2olo
From Proteopedia
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'''NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: open form at 1.9A resolution''' | '''NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: open form at 1.9A resolution''' | ||
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[[Category: Venci, D.]] | [[Category: Venci, D.]] | ||
[[Category: Zhao, G.]] | [[Category: Zhao, G.]] | ||
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| - | [[Category: | + | [[Category: Oxidoreductase]] |
| - | [[Category: | + | [[Category: Rossmann fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:10:12 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:10, 4 May 2008
NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: open form at 1.9A resolution
Overview
NikD is an unusual amino-acid-oxidizing enzyme that contains covalently bound FAD, catalyzes a 4-electron oxidation of piperideine-2-carboxylic acid to picolinate, and plays a critical role in the biosynthesis of nikkomycin antibiotics. Crystal structures of closed and open forms of nikD, a two-domain enzyme, have been determined to resolutions of 1.15 and 1.9 A, respectively. The two forms differ by an 11 degrees rotation of the catalytic domain with respect to the FAD-binding domain. The active site is inaccessible to solvent in the closed form; an endogenous ligand, believed to be picolinate, is bound close to and parallel with the flavin ring, an orientation compatible with redox catalysis. The active site is solvent accessible in the open form, but the picolinate ligand is approximately perpendicular to the flavin ring and a tryptophan is stacked above the flavin ring. NikD also contains a mobile cation binding loop.
About this Structure
2OLO is a Single protein structure of sequence from Streptomyces tendae. Full crystallographic information is available from OCA.
Reference
NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: structures of closed and open forms at 1.15 and 1.90 A resolution., Carrell CJ, Bruckner RC, Venci D, Zhao G, Jorns MS, Mathews FS, Structure. 2007 Aug;15(8):928-41. PMID:17697998 Page seeded by OCA on Sun May 4 11:10:12 2008
