2hgh
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(New page: 200px<br /> <applet load="2hgh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hgh" /> '''Transcription Factor IIIA zinc fingers 4-6 ...)
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Revision as of 07:01, 18 November 2007
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Transcription Factor IIIA zinc fingers 4-6 bound to 5S rRNA 55mer (NMR structure)
Overview
Transcription factor IIIA (TFIIIA) is a Cys2His2 zinc finger protein that, regulates expression of the 5 S ribosomal RNA gene by binding specifically, to the internal control element. TFIIIA also functions in transport and, storage of 5 S RNA by binding directly to the RNA transcript. To obtain, insights into the mechanism by which TFIIIA recognizes 5 S RNA, we, determined the solution structure of the middle three zinc fingers bound, to the central core of 5 S RNA. Finger 4 utilizes "lock and key", recognition to bind in the widened major groove of the pre-structured RNA, loop E motif. This interaction is mediated by direct hydrogen bonding, interactions with bases. In contrast, recognition of loop A, a flexible, junction of three helices, occurs by an induced fit mechanism that, involves reorganization of the conserved CAUA motif and structuring of the, finger 5-finger 6 interface to form a complementary RNA binding surface.
About this Structure
2HGH is a Single protein structure of sequence from Xenopus laevis with ZN as ligand. The following page contains interesting information on the relation of 2HGH with [Zinc Fingers]. Full crystallographic information is available from OCA.
Reference
Induced fit and "lock and key" recognition of 5S RNA by zinc fingers of transcription factor IIIA., Lee BM, Xu J, Clarkson BK, Martinez-Yamout MA, Dyson HJ, Case DA, Gottesfeld JM, Wright PE, J Mol Biol. 2006 Mar 17;357(1):275-91. Epub 2005 Dec 20. PMID:16405997
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