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2opc
From Proteopedia
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[[Image:2opc.gif|left|200px]] | [[Image:2opc.gif|left|200px]] | ||
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'''Structure of Melampsora lini avirulence protein, AvrL567-A''' | '''Structure of Melampsora lini avirulence protein, AvrL567-A''' | ||
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[[Category: Teh, T.]] | [[Category: Teh, T.]] | ||
[[Category: Wang, C I.]] | [[Category: Wang, C I.]] | ||
| - | [[Category: | + | [[Category: Avrl567-a]] |
| - | [[Category: | + | [[Category: Cobalt]] |
| - | [[Category: | + | [[Category: Crystallization]] |
| - | [[Category: | + | [[Category: Plant disease resistance]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:22:53 2008'' | |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:22, 4 May 2008
Structure of Melampsora lini avirulence protein, AvrL567-A
Overview
Metal-binding sites are ubiquitous in proteins and can be readily utilized for phasing. It is shown that a protein crystal structure can be solved using single-wavelength anomalous diffraction based on the anomalous signal of a cobalt ion measured on a conventional monochromatic X-ray source. The unique absorption edge of cobalt (1.61 A) is compatible with the Cu K alpha wavelength (1.54 A) commonly available in macromolecular crystallography laboratories. This approach was applied to the determination of the structure of Melampsora lini avirulence protein AvrL567-A, a protein with a novel fold from the fungal pathogen flax rust that induces plant disease resistance in flax plants. This approach using cobalt ions may be applicable to all cobalt-binding proteins and may be advantageous when synchrotron radiation is not readily available.
About this Structure
2OPC is a Single protein structure of sequence from Melampsora lini. Full crystallographic information is available from OCA.
Reference
The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein., Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):209-13. Epub 2007 Feb 23. PMID:17329816 Page seeded by OCA on Sun May 4 11:22:53 2008
