2oqp
From Proteopedia
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'''Solution structure of human interleukin-21''' | '''Solution structure of human interleukin-21''' | ||
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[[Category: Bondensgaard, K.]] | [[Category: Bondensgaard, K.]] | ||
[[Category: Breinholt, J.]] | [[Category: Breinholt, J.]] | ||
| - | [[Category: | + | [[Category: Cytokine]] |
| - | [[Category: | + | [[Category: Four helix bundle]] |
| - | [[Category: | + | [[Category: Multiple conformer]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:28:26 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:28, 4 May 2008
Solution structure of human interleukin-21
Overview
The high resolution three-dimensional structure of human interleukin (hIL)-21 has been resolved by heteronuclear NMR spectroscopy. Overall, the hIL-21 structure is dominated by a well defined central four-helical bundle, arranged in an up-up-down-down topology, as observed for other cytokines. A segment of the hIL-21 molecule that includes the third helical segment, helix C, is observed to exist in two distinct and interchangeable states. In one conformer, the helix C segment is presented in a regular, alpha-helical conformation, whereas in the other conformer, this segment is largely disordered. A structure-based sequence alignment of hIL-21 with receptor complexes of the related cytokines, interleukin-2 and -4, implied that this particular segment is involved in receptor binding. An hIL-21 analog was designed to stabilize the region around helix C through the introduction of a segment grafted from hIL-4. This novel hIL-21 analog was demonstrated to exhibit a 10-fold increase in potency in a cellular assay.
About this Structure
2OQP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The existence of multiple conformers of interleukin-21 directs engineering of a superpotent analogue., Bondensgaard K, Breinholt J, Madsen D, Omkvist DH, Kang L, Worsaae A, Becker P, Schiodt CB, Hjorth SA, J Biol Chem. 2007 Aug 10;282(32):23326-36. Epub 2007 Jun 12. PMID:17565991 Page seeded by OCA on Sun May 4 11:28:26 2008
