2ost
From Proteopedia
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[[Image:2ost.gif|left|200px]] | [[Image:2ost.gif|left|200px]] | ||
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'''The structure of a bacterial homing endonuclease : I-Ssp6803I''' | '''The structure of a bacterial homing endonuclease : I-Ssp6803I''' | ||
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[[Category: Stoddard, B L.]] | [[Category: Stoddard, B L.]] | ||
[[Category: Zhao, L.]] | [[Category: Zhao, L.]] | ||
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| - | [[Category: | + | [[Category: Homing endonuclease]] |
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Revision as of 08:35, 4 May 2008
The structure of a bacterial homing endonuclease : I-Ssp6803I
Overview
The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.
About this Structure
2OST is a Single protein structure of sequence from Synechocystis sp.. Full crystallographic information is available from OCA.
Reference
The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif., Zhao L, Bonocora RP, Shub DA, Stoddard BL, EMBO J. 2007 May 2;26(9):2432-42. Epub 2007 Apr 5. PMID:17410205 Page seeded by OCA on Sun May 4 11:35:07 2008
